Yamahira Shinya, Yamaguchi Satoshi, Kawahara Masahiro, Nagamune Teruyuki
Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan.
Macromol Biosci. 2014 Dec;14(12):1670-6. doi: 10.1002/mabi.201400312. Epub 2014 Sep 5.
Cell patterning on photo-responsive materials are a promising tool for preparing unique single-cell arrays. However, most conventional single-cell arrays on such smart materials can be applied only to adherent cells and limit cellular functions such as extension and migration within the patterned adhesive surfaces. In this study, a versatile single cell array that works with both non-adherent and adherent cells was constructed using a photo-cleavable polyethylene glycol (PEG)-lipid/collagen surface. On this single-cell array, cells behaved similar to their native functions without limitation from the patterned surface. Furthermore, quantitative imaging analyses of cellular motility and morphological changes were performed in a high-throughput manner.
在光响应材料上进行细胞图案化是制备独特单细胞阵列的一种有前途的工具。然而，这种智能材料上的大多数传统单细胞阵列仅适用于贴壁细胞，并限制了细胞功能，如在图案化粘附表面内的伸展和迁移。在本研究中，使用可光裂解的聚乙二醇（PEG）-脂质/胶原蛋白表面构建了一种适用于非贴壁细胞和贴壁细胞的通用单细胞阵列。在这种单细胞阵列上，细胞表现出与其天然功能相似的行为，不受图案化表面的限制。此外，还以高通量方式对细胞运动性和形态变化进行了定量成像分析。
