Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 226-8501, Japan.
Chem Commun (Camb). 2013 Jul 21;49(57):6394-6. doi: 10.1039/c3cc41632a.
We developed a novel peptide array format incorporating a photo-cleavable linker for monitoring cellular uptake. Model peptides were successfully immobilised via the photo-cleavable linker onto conventional plates and could be released spatiotemporally using UV irradiation. Incorporation of confocal microscopy allowed for detailed real-time monitoring of cellular internalisation of peptides.
我们开发了一种新型的肽阵列格式，其中包含一个光可裂解的连接子，用于监测细胞摄取。模型肽通过光可裂解的连接子成功地固定在常规板上，并可以使用紫外线照射进行时空释放。共聚焦显微镜的结合允许对肽的细胞内化进行详细的实时监测。
