采用手动标记方法制备用于小动物正电子发射断层显像（PET）成像的⁶⁸Ga标记的DOTA肽。
Preparation of ⁶⁸Ga-labelled DOTA-peptides using a manual labelling approach for small-animal PET imaging.
作者信息
Romero Eduardo, Martínez Alfonso, Oteo Marta, García Angel, Morcillo Miguel Angel
机构信息
CIEMAT - Research Centre for Energy, Environment and Technology, Biomedical Applications and Pharmacokinetics Unit, Avenida Complutense 40, 28040 Madrid, Spain.
CIEMAT - Research Centre for Energy, Environment and Technology, Biomedical Applications and Pharmacokinetics Unit, Avenida Complutense 40, 28040 Madrid, Spain.
出版信息
Appl Radiat Isot. 2016 Jan;107:113-120. doi: 10.1016/j.apradiso.2015.10.005. Epub 2015 Oct 13.
(68)Ga-DOTA-peptides are a promising PET radiotracers used in the detection of different tumours types due to their ability for binding specifically receptors overexpressed in these. Furthermore, (68)Ga can be produced by a (68)Ge/(68)Ga generator on site which is a very good alternative to cyclotron-based PET isotopes. Here, we describe a manual labelling approach for the synthesis of (68)Ga-labelled DOTA-peptides based on concentration and purification of the commercial (68)Ga/(68)Ga generator eluate using an anion exchange-cartridge. (68)Ga-DOTA-TATE was used to image a pheochromocytoma xenograft mouse model by a microPET/CT scanner. The method described provides satisfactory results, allowing the subsequent (68)Ga use to label DOTA-peptides. The simplicity of the method along with its implementation reduced cost, makes it useful in preclinical PET studies.
（68）Ga-DOTA肽是一种很有前景的PET放射性示踪剂，由于它们能够特异性结合在不同肿瘤类型中过表达的受体，因而可用于检测这些肿瘤。此外，（68）Ga可通过现场的（68）Ge/（68）Ga发生器产生，这是基于回旋加速器的PET同位素的一个很好的替代方案。在此，我们描述了一种手动标记方法，用于基于使用阴离子交换柱对商用（68）Ga/（68）Ga发生器洗脱液进行浓缩和纯化来合成（68）Ga标记的DOTA肽。（68）Ga-DOTA-TATE用于通过微型PET/CT扫描仪对嗜铬细胞瘤异种移植小鼠模型进行成像。所描述的方法提供了令人满意的结果，使得随后能够使用（68）Ga标记DOTA肽。该方法的简单性及其实施成本的降低，使其在临床前PET研究中很有用。
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