包括阳离子交换预纯化在内的DOTA肽（68）Ga标记的全自动化。
Full automation of (68)Ga labelling of DOTA-peptides including cation exchange prepurification.
作者信息
Ocak M, Antretter M, Knopp R, Kunkel F, Petrik M, Bergisadi N, Decristoforo C
机构信息
Clinical Department of Nuclear Medicine, Medical University Innsbruck, Anichstrasse 35, A-6020 Innsbruck, Austria.
出版信息
Appl Radiat Isot. 2010 Feb;68(2):297-302. doi: 10.1016/j.apradiso.2009.10.006. Epub 2009 Oct 24.
Here we describe a fully automated approach for the synthesis of (68)Ga-labelled DOTA-peptides based on pre-concentration and purification of the generator eluate by using a cation exchange-cartridge and its comparison with fully automated direct labelling applying fractionated elution. Pre-concentration of the eluate on a cation exchange cartridge both using a resin-based and a disposable cation-exchange cartridge efficiently removed (68)Ge as well as major metal contaminations with Fe and Zn. This resulted in a high labelling efficiency of DOTA-peptides at high specific activity (SA) with short synthesis times.
在此，我们描述了一种基于使用阳离子交换柱对发生器洗脱液进行预浓缩和纯化来合成（68）Ga标记的DOTA肽的全自动方法，并将其与采用分步洗脱的全自动直接标记法进行比较。使用基于树脂的阳离子交换柱和一次性阳离子交换柱对洗脱液进行预浓缩，均能有效去除（68）Ge以及铁和锌等主要金属污染物。这使得DOTA肽在高比活度（SA）下具有较高的标记效率，且合成时间较短。
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