Meyer G-J, Mäcke H, Schuhmacher J, Knapp W H, Hofmann M
Klinik für Nuklearmedizin, Medizinische Hochschule Hannover, Carl Neuberg Str.1, 30623, Hannover, Germany.
Eur J Nucl Med Mol Imaging. 2004 Aug;31(8):1097-104. doi: 10.1007/s00259-004-1486-0. Epub 2004 Mar 17.
68Ge/68Ga generators provide cyclotron-independent access to positron emission tomography (PET) radiopharmaceuticals. We describe a system which allows the safe and efficient handling of 68Ge/68Ga generator eluates for labelling of DOTA-derivatised peptide ligands. The system comprises concentration and purification of the 68Ga eluate as well as labelling and purification steps for peptides, and can be used with different 68Ge/68Ga generator types. The suitability and efficiency were tested with two different DOTA-derivatised somatostatin derivatives and a DOTA-derivatised bombesin derivative. Amounts of 10-20 nmol of the peptides were sufficient and resulted in labelling yields of 50% for all peptides. The built-in safety precautions have proven to be appropriate in allowing use of the method for routine clinical applications. The system was set up and operated in a "hot lab" by personnel with no previous experience in the preparation of PET radiopharmaceuticals.
68Ge/68Ga发生器提供了无需回旋加速器即可获得正电子发射断层扫描（PET）放射性药物的途径。我们描述了一种系统，该系统能够安全、高效地处理68Ge/68Ga发生器洗脱液，用于标记DOTA衍生的肽配体。该系统包括68Ga洗脱液的浓缩和纯化以及肽的标记和纯化步骤，并且可与不同类型的68Ge/68Ga发生器一起使用。使用两种不同的DOTA衍生的生长抑素衍生物和一种DOTA衍生的蛙皮素衍生物对该系统的适用性和效率进行了测试。10 - 20 nmol的肽量就足够了，并且所有肽的标记产率均为50%。已证明内置的安全预防措施适用于允许该方法用于常规临床应用。该系统由之前没有PET放射性药物制备经验的人员在“热室”中设置和操作。
