Matthews D R, Hosker J P
Diabetes Research Laboratories, Radcliffe Infirmary, Oxford, United Kingdom.
Diabetes Care. 1989 Feb;12(2):156-9. doi: 10.2337/diacare.12.2.156.
Glucose clamping has become a widely used test for assessing insulin sensitivity and beta-cell function. We present a new method of achieving a reliable and unbiased clamp using an iterative computer program. After initial parameter estimation, the program uses no fixed algorithm or physiological preconceptions but makes predictions according to previous glycemic responses to infusion rates. The program can be used for human or animal studies and for euglycemic or hyperglycemic clamps. The program has flexible data entry, graphic display, running statistics of mean infusion data, and mean glucose levels and their standard deviations. These form part of the criteria for the assessment of glucose clamping, which includes the sampling interval, the duration of the clamp, the achieved glucose concentration (together with its coefficient of variation), and the percent change of glucose infused at the end of the clamp. Data for 151 consecutive clamp studies were analyzed. Median coefficient of variation of glucose values at the end of the clamp was 2%.
葡萄糖钳夹技术已成为评估胰岛素敏感性和β细胞功能的一种广泛应用的检测方法。我们提出了一种使用迭代计算机程序实现可靠且无偏差钳夹的新方法。在初始参数估计之后，该程序不使用固定算法或生理先入之见，而是根据先前对输注速率的血糖反应进行预测。该程序可用于人体或动物研究以及正常血糖或高血糖钳夹。该程序具有灵活的数据输入、图形显示、平均输注数据的运行统计以及平均血糖水平及其标准差。这些构成了评估葡萄糖钳夹的标准的一部分，评估标准包括采样间隔、钳夹持续时间、达到的葡萄糖浓度（及其变异系数）以及钳夹结束时输注葡萄糖的百分比变化。对151项连续钳夹研究的数据进行了分析。钳夹结束时葡萄糖值的中位变异系数为2%。
