Heinemann L, Ampudia-Blasco F J
Klinik für Stoffwechselkrankheiten und Ernährung (WHO Collaborating Center for Diabetes), Heinrich-Heine-Universität Düsseldorf, Germany.
Horm Metab Res. 1994 Dec;26(12):579-83. doi: 10.1055/s-2007-1001763.
The Biostator makes it possible to perform glucose clamp experiments almost automatically. Thus, blood glucose concentrations can be maintained at (or close to) a target level with substantially less effort than with the manual clamp technique. The automatisation also avoids a potential bias on the part of the investigator. However, as with the non-automated manual clamp technique, blood glucose concentrations do not remain exactly at the target value, as they show a considerable scatter around the target value. This scatter is generated by the time constants of the Biostator, i.e. the whole closed-loop system, and the autoregressive properties of the glucose clamp algorithm used. In order to describe the quality of glucose clamps over time more precisely, "cumulative sums" as an alternative to the usual coefficient of variation can be used. Practical work with the Biostator is burdened with technical difficulties and considerable costs in comparison to the manual clamp technique. Deficits concerning data storage and presentation capability of the Biostator have been overcome by an appropriate programme for an external computer. The use of the Biostator for the glucose clamp technique is not mandatory, but, the use of this machine makes it possible to perform glucose clamp studies under standardised and reproducible conditions.
生物定标器使得几乎可以自动进行葡萄糖钳夹实验。因此，与手动钳夹技术相比，维持血糖浓度在（或接近）目标水平所需的工作量大幅减少。自动化操作还避免了研究人员可能存在的偏差。然而，与非自动化的手动钳夹技术一样，血糖浓度并不会精确地保持在目标值，而是在目标值周围有相当大的波动。这种波动是由生物定标器（即整个闭环系统）的时间常数以及所使用的葡萄糖钳夹算法的自回归特性产生的。为了更精确地描述葡萄糖钳夹随时间的质量，可以使用“累积和”来替代通常的变异系数。与手动钳夹技术相比，使用生物定标器进行实际操作存在技术困难且成本高昂。通过为外部计算机编写的适当程序，已经克服了生物定标器在数据存储和呈现能力方面的不足。使用生物定标器进行葡萄糖钳夹技术并非强制要求，但使用这台机器能够在标准化和可重复的条件下进行葡萄糖钳夹研究。
