Herlihy Sarah E, Starke Hannah E, Lopez-Anton Melisa, Cox Nehemiah, Keyhanian Katayoon, Fraser Donald J, Gomer Richard H
Texas A&M University, College Station, TX, USA.
School of Medicine, Cardiff University, Cardiff, Wales, United Kingdom.
Perit Dial Int. 2016 Jul-Aug;36(4):367-73. doi: 10.3747/pdi.2014.00284. Epub 2015 Oct 22.
Long-term peritoneal dialysis (PD) often results in the development of peritoneal fibrosis. In many other fibrosing diseases, monocytes enter the fibrotic lesion and differentiate into fibroblast-like cells called fibrocytes. We find that peritoneal tissue from short-term PD patients contains few fibrocytes, while fibrocytes are readily observed in the peritoneal membrane of long-term PD patients. The PD fluid Dianeal (Baxter Healthcare Corporation, Deerfield, IL, USA) contains dextrose, a number of electrolytes including sodium chloride, and sodium lactate. We find that PD fluid potentiates human fibrocyte differentiation in vitro and implicates sodium lactate in this potentiation. The plasma protein serum amyloid P (SAP) inhibits fibrocyte differentiation. Peritoneal dialysis fluid and sodium chloride decrease the ability of human SAP to inhibit human fibrocyte differentiation in vitro Together, these results suggest that PD fluid contributes to the development of peritoneal fibrosis by potentiating fibrocyte differentiation.
长期腹膜透析（PD）常导致腹膜纤维化。在许多其他纤维化疾病中，单核细胞进入纤维化病灶并分化为成纤维细胞样细胞，即纤维细胞。我们发现，短期PD患者的腹膜组织中纤维细胞很少，而在长期PD患者的腹膜中很容易观察到纤维细胞。腹膜透析液Dianeal（美国伊利诺伊州迪尔菲尔德市百特医疗保健公司）含有葡萄糖、多种电解质，包括氯化钠和乳酸钠。我们发现腹膜透析液在体外可增强人纤维细胞的分化，并表明乳酸钠参与了这种增强作用。血浆蛋白血清淀粉样蛋白P（SAP）可抑制纤维细胞分化。腹膜透析液和氯化钠在体外可降低人SAP抑制人纤维细胞分化的能力。这些结果共同表明，腹膜透析液通过增强纤维细胞分化促进腹膜纤维化的发展。
