Bourbeau P, Dicker D, Higgins M L, Daneo-Moore L
Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.
J Bacteriol. 1989 Apr;171(4):1982-6. doi: 10.1128/jb.171.4.1982-1986.1989.
Cultures of Enterococcus faecium growing at various rates were examined for timing of cell division cycle events by using the method of residual divisions and a morphological analysis. Both methods gave essentially the same timing for the onset of D1 (completion of chromosome replication) and of D2 (completion of septation). Frequencies of cells exhibiting a phase-reversed center in bovine serum albumin at various growth rates were determined. The data fit a model in which rapidly growing cells increase in refractive index (which is assumed to represent central density) at completion of the chromosome replication cycle involved in the ongoing division, whereas slowly growing cultures increase in central density at the time of completion of septation. There was no correlation between the timing of increase in central density and the timing of initiation of new sites of surface growth.
采用剩余分裂法和形态学分析方法，对以不同速率生长的粪肠球菌培养物进行细胞分裂周期事件的时间检测。两种方法得出的D1期（染色体复制完成）和D2期（隔膜形成完成）开始的时间基本相同。测定了不同生长速率下在牛血清白蛋白中呈现相位反转中心的细胞频率。数据符合一个模型，即快速生长的细胞在正在进行的分裂所涉及的染色体复制周期完成时，折射率（假定代表中心密度）增加，而缓慢生长的培养物在隔膜形成完成时中心密度增加。中心密度增加的时间与表面新生长位点起始的时间之间没有相关性。
