枯草芽孢杆菌细胞周期中的浮力密度波动。

Buoyant density fluctuations during the cell cycle of Bacillus subtilis.

作者信息

Hart A, Edwards C

出版信息

Arch Microbiol. 1987 Feb;147(1):68-72. doi: 10.1007/BF00492907.

Abstract

A simple rapid method for preparing synchronous cultures of Bacillus subtilis has been used to investigate changes in density during the cell cycle. Asynchronous cells separated on a stepped Percoll density gradient had a mean cell density of 1.117 g ml-1 +/- 0.004. Samples from a synchronous culture exhibited variation (ca. 1.5%) in mean cell density which was greatest at the onset of cell division. An asynchronous control culture showed little variation in density. These results are discussed in relation to previous work on Escherichia coli.

摘要

一种用于制备枯草芽孢杆菌同步培养物的简单快速方法已被用于研究细胞周期中密度的变化。在阶梯状 Percoll 密度梯度上分离的非同步细胞的平均细胞密度为 1.117 g/ml ± 0.004。同步培养物的样本在平均细胞密度上表现出变化（约 1.5%），这种变化在细胞分裂开始时最大。非同步对照培养物的密度变化很小。这些结果结合先前关于大肠杆菌的研究进行了讨论。