Chu C C, Templin A, Clark A J
Department of Molecular Biology, University of California, Berkeley 94720.
J Bacteriol. 1989 Apr;171(4):2101-9. doi: 10.1128/jb.171.4.2101-2109.1989.
The nucleotide sequences of a small gene, racC, and the adjacent N-terminal half of the wild-type recE gene are presented. A frameshift mutation, recE939, inactivating recE and preventing synthesis of the active recE enzyme, exonuclease VIII, was identified. The endpoints of five deletion mutations suppressing recE939 were sequenced. All five delete the frameshift site. Two are intra-recE deletions and fuse the N- and C-terminal portions of recE in frame. Three of the deletions remove the entire N-terminal portion of recE, fusing the C-terminal portion to N-terminal portions of racC in frame. These data indicate that about 70% of the N-terminal half of recE is not required to encode a hypothesized protein domain with exonuclease VIII activity.
本文展示了一个小基因racC以及野生型recE基因相邻的N端一半的核苷酸序列。鉴定出一种移码突变recE939，它使recE失活并阻止活性recE酶（核酸外切酶VIII）的合成。对抑制recE939的五个缺失突变的端点进行了测序。所有五个突变都删除了移码位点。其中两个是recE内部缺失，并使recE的N端和C端部分框内融合。三个缺失突变删除了recE的整个N端部分，使C端部分与racC的N端部分框内融合。这些数据表明，recE N端一半中约70%对于编码具有核酸外切酶VIII活性的假定蛋白结构域不是必需的。
