Zhao Fengli, Ma Jianhui, Li Libei, Fan Shuli, Guo Yaning, Song Meizhen, Wei Hengling, Pang Chaoyou, Yu Shuxun
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan, PR China; Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, Guangdong, PR China.
College of Life Sciences, Henan Normal University, Xinxiang, Henan, PR China.
Gene. 2016 Jan 15;576(1 Pt 2):268-74. doi: 10.1016/j.gene.2015.10.042. Epub 2015 Oct 20.
NAC (NAM, ATAF, and CUC) is one of the largest transcription factor families in plants, and its members play various roles in plant growth, development, and the response to biotic and abiotic stresses. Currently, 77 NAC genes have been reported in cotton (Gossypium hirsutum L.). And GhNAC12 showed up-regulation during leaf senescence, but its role in this process is poorly understood. In the present study, a preliminary function analysis of GhNAC12 was performed during leaf senescence. qRT-PCR analysis indicated that GhNAC12 expression increased during the early-aging process and the aging of cotyledons. Additionally, we observed that overexpression of GhNAC12 in Arabidopsis led to early senescence (early aging). Our findings suggest that GhNAC12 is a candidate gene for early aging in upland cotton cultivars. Neutrality tests suggested that there was no selection pressure imposed on GhNAC12 during the domestication of upland cotton.
NAC（NAM、ATAF和CUC）是植物中最大的转录因子家族之一，其成员在植物生长、发育以及对生物和非生物胁迫的响应中发挥着各种作用。目前，棉花（陆地棉）中已报道了77个NAC基因。GhNAC12在叶片衰老过程中表现出上调，但对其在该过程中的作用了解甚少。在本研究中，对GhNAC12在叶片衰老过程中进行了初步功能分析。qRT-PCR分析表明，GhNAC12的表达在子叶早衰过程和衰老过程中增加。此外，我们观察到在拟南芥中过表达GhNAC12会导致早衰（早期衰老）。我们的研究结果表明，GhNAC12是陆地棉品种早衰的候选基因。中性检验表明，在陆地棉驯化过程中，GhNAC12没有受到选择压力。
