Wang Chenlei, Li Tengyu, Liu Qibao, Li Libei, Feng Zhen, Yu Shuxun
The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, College of Advanced Agricultural Sciences, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China.
State Key Laboratory of Cotton Biology, Institute of Cotton Research of the Chinese Academy of Agricultural Sciences, Anyang 455000, China.
Plants (Basel). 2022 Jun 1;11(11):1491. doi: 10.3390/plants11111491.
In the process of growth and development, cotton exhibits premature senescence under various abiotic stresses, impairing yield and fiber quality. NAC (NAM, ATAF1,2, and CUC2) protein widely distributed in land plants, play the critical role in responding to abiotic stress and regulating leaf senescence. We have identified and functional analyzed a NAM domain gene in upland cotton, it was located on the A11 chromosome 4,921,702 to 4,922,748 bp, only containing one exon. The spatio-temporal expression pattern analysis revealed that it was highly expressed in root, torus, ovule and fiber development stage. The results of qRT-PCR validated that negatively regulated by salt stress, drought stress, HO stress, IAA treatment, and ethylene treatment, positively regulated by the ABA and MeJA treatment. Moreover, heterologous overexpression of results in leaf premature senescence and delays root system development in . The phenotype of delayed-senescence was performed after silencing by VIGS in premature cotton. Taken together, was involved in different abiotic stress pathways and play important roles in negatively regulating leaf premature senescence.
在生长发育过程中，棉花在各种非生物胁迫下会出现早衰现象，从而影响产量和纤维品质。NAC（NAM、ATAF1、2和CUC2）蛋白广泛分布于陆生植物中，在应对非生物胁迫和调节叶片衰老方面发挥着关键作用。我们已经在陆地棉中鉴定并对一个NAM结构域基因进行了功能分析，它位于A11染色体上4,921,702至4,922,748 bp处，仅包含一个外显子。时空表达模式分析表明，它在根、托叶、胚珠和纤维发育阶段高度表达。qRT-PCR结果验证了其受盐胁迫、干旱胁迫、HO胁迫、IAA处理和乙烯处理的负调控，受ABA和MeJA处理的正调控。此外，该基因的异源过表达导致拟南芥叶片早衰并延迟根系发育。通过VIGS在早衰棉花中沉默该基因后，出现了延缓衰老的表型。综上所述，该基因参与了不同的非生物胁迫途径，并在负调控叶片早衰中发挥重要作用。
