Liu Zhanji, Fu Mingchuan, Li Hao, Chen Yizhen, Wang Liguo, Liu Renzhong
Key Laboratory of Cotton Breeding and Cultivation in Huang-Huai-Hai Plain, Ministry of Agriculture, Cotton Research Center of Shandong Academy of Agricultural Sciences, Jinan, China.
PeerJ. 2019 Nov 5;7:e7995. doi: 10.7717/peerj.7995. eCollection 2019.
As one of the largest plant-specific gene families, the NAC transcription factor gene family plays important roles in various plant physiological processes that are related to plant development, hormone signaling, and biotic and abiotic stresses. However, systematic investigation of the gene family in sea-island cotton ( L.) has not been reported, to date. The recent release of the complete genome sequence of sea-island cotton allowed us to perform systematic analyses of ) genes. In this study, we performed a genome-wide survey and identified 270 genes in the sea-island cotton genome. Genome mapping analysis showed that genes were unevenly distributed on 26 chromosomes. Through phylogenetic analyses of GbNACs along with their Arabidopsis counterparts, these proteins were divided into 10 groups (I-X), and each contained a different number of GbNACs with a similar gene structure and conserved motifs. One hundred and fifty-four duplicated gene pairs were identified, and almost all of them exhibited strong purifying selection during evolution. In addition, various -acting regulatory elements in genes were found to be related to major hormones, defense and stress responses. Notably, transcriptome data analyses unveiled the expression profiles of 62 genes under Verticillium wilt (VW) stress. Furthermore, the expression profiles of 15 genes tested by quantitative real-time PCR (qPCR) demonstrated that they were sensitive to methyl jasmonate (MeJA) and salicylic acid (SA) treatments and that they could be involved in pathogen-related hormone regulation. Taken together, the genome-wide identification and expression profiling pave new avenues for systematic functional analysis of candidates, which may be useful for improving cotton defense against VW.
作为最大的植物特异性基因家族之一，NAC转录因子基因家族在与植物发育、激素信号传导以及生物和非生物胁迫相关的各种植物生理过程中发挥着重要作用。然而，迄今为止，尚未见关于海岛棉（Gossypium barbadense L.）中该基因家族的系统研究报道。海岛棉完整基因组序列的最新公布使我们能够对GbNAC基因进行系统分析。在本研究中，我们进行了全基因组调查，在海岛棉基因组中鉴定出270个GbNAC基因。基因组图谱分析表明，这些基因不均匀地分布在26条染色体上。通过对GbNACs及其拟南芥对应物进行系统发育分析，这些蛋白质被分为10组（I - X），每组包含不同数量的GbNACs，具有相似的基因结构和保守基序。鉴定出154对重复基因对，并且几乎所有这些基因对在进化过程中都表现出强烈的纯化选择。此外，发现GbNAC基因中的各种顺式作用调控元件与主要激素、防御和胁迫反应相关。值得注意的是，转录组数据分析揭示了62个GbNAC基因在黄萎病（VW）胁迫下的表达谱。此外，通过定量实时PCR（qPCR）检测的15个GbNAC基因的表达谱表明，它们对茉莉酸甲酯（MeJA）和水杨酸（SA）处理敏感，并且可能参与病原体相关激素调节。综上所述，全基因组鉴定和表达谱分析为GbNAC候选基因的系统功能分析开辟了新途径，这可能有助于提高棉花对黄萎病的抗性。
