Hong Keqian, Gong Deqiang, Zhang Lubin, Hu Huigang, Jia Zhiwei, Gu Hui, Song Kanghua
Key Laboratory for Postharvest Physiology and Technology of Tropical Horticultural Products of Hainan Province, South Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, Guangdong, China.
Key Laboratory for Postharvest Physiology and Technology of Tropical Horticultural Products of Hainan Province, South Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, Guangdong, China.
Gene. 2016 Jan 15;576(1 Pt 2):275-83. doi: 10.1016/j.gene.2015.10.041. Epub 2015 Oct 20.
Anthracnose, caused by Colletotrichum gloeosporioides, is a major disease of the postharvest mango (Mangifera indica L.) fruit. However, a lack of transcriptomic and genomic information hinders our understanding of the molecular mechanisms underlying the mango fruit defense response. Here, we studied the host responses of the mango fruit against C. gloeosporioides using Illumina paired-end sequencing technology, and expression profiles of 35 defense-related genes were further analyzed by qRT-PCR. The results indicated that 5.9Gigabase pair clean reads were assembled into a total of 131,750 unigenes, of which 89,050 unigenes found to be homologous to genes in the NCBI GenBank database and 61,694 unigenes annonated in the Swiss-Prot database. Orthologous analyses showed that 47,770 unigenes were assigned with one or more Gene Ontology terms and 44,145 unigenes were classified into 256 Kyoto Encyclopedia of Genes and Genomes pathways. Moreover, qRT-PCR of 35 defense-related unigenes, including 17 ethylene response factors (ERFs), 6 nucleotide binding site-leucine-rich repeats (NBS-LRRs), 6 nonexpressor of pathogenesis-related genes (NPRs) and 6 pathogenesis-related protein (PRs), revealed that most of these defense-related genes were up-regulated after C. gloeosporioides infection. Taken together, our study provides a platform to discover new candidate genes in mango fruit in relation to pathogen resistance.
由胶孢炭疽菌引起的炭疽病是芒果（Mangifera indica L.）采后果实的一种主要病害。然而，转录组和基因组信息的缺乏阻碍了我们对芒果果实防御反应潜在分子机制的理解。在此，我们利用Illumina双末端测序技术研究了芒果果实对胶孢炭疽菌的宿主反应，并通过qRT-PCR进一步分析了35个与防御相关基因的表达谱。结果表明，59亿碱基对的干净 reads 被组装成总共131,750个单基因，其中89,050个单基因被发现与NCBI GenBank数据库中的基因同源，61,694个单基因在Swiss-Prot数据库中被注释。直系同源分析表明，47,770个单基因被赋予一个或多个基因本体论术语，44,145个单基因被分类到256条京都基因与基因组百科全书途径中。此外，对35个与防御相关的单基因进行qRT-PCR分析，包括17个乙烯反应因子（ERF）、6个核苷酸结合位点富含亮氨酸重复序列（NBS-LRR）、6个病程相关基因非表达子（NPR）和6个病程相关蛋白（PR），结果显示，这些与防御相关的基因在胶孢炭疽菌感染后大多上调。综上所述，我们的研究提供了一个平台，用于发现芒果果实中与抗病性相关的新候选基因。
