State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University , Yangling, Shaanxi, China.
Institute of Plant Protection, Guangxi Academy of Agricultural Sciences, Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs and Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests , Nanning, Guangxi, China.
mBio. 2023 Aug 31;14(4):e0062923. doi: 10.1128/mbio.00629-23. Epub 2023 Jun 7.
Anthracnose diseases caused by species are among the most common fungal diseases. These symptoms typically manifest as dark, sunken lesions on leaves, stems, and fruit. In China, mango anthracnose seriously affects fruit yield and quality. Genome sequencing of several species shows the presence of mini-chromosomes. These are thought to contribute to virulence, but their formation and activity remain to be fully elucidated. Here, we assembled 17 genomes (16 isolated from mango plus one from persimmon) through PacBio long-read sequencing. Half of the assembled scaffolds had telomeric repeats at both ends indicating full-length chromosomes. Based on comparative genomics analysis at interspecies and intraspecies levels, we identified extensive chromosomal rearrangements events. We analyzed mini-chromosomes of spp. and found large variation among close relatives. In , homology between core chromosomes and mini-chromosomes suggested that some mini-chromosomes were generated by recombination of core chromosomes. In GZ23-3, we found 26 horizontally transferred genes arranged in clusters on mini-chromosomes. In FJ11-1, several potential pathogenesis-related genes on mini-chromosomes were upregulated, especially in strains with highly pathogenic phenotypes. Mutants of these upregulated genes showed obvious defects in virulence. Our findings provide insights into the evolution and potential relationships to virulence associated with mini-chromosomes. IMPORTANCE is a cosmopolitan fungal genus that seriously affects fruit yield and quality of many plant species. Mini-chromosomes have been found to be related to virulence in . Further examination of mini-chromosomes can help us elucidate some pathogenic mechanisms of . In this study, we generated novel assemblies of several strains. Comparative genomic analyses within and between species were conducted. We then identified mini-chromosomes in our sequenced strains systematically. The characteristics and generation of mini-chromosomes were investigated. Transcriptome analysis and gene knockout revealed pathogenesis-related genes located on mini-chromosomes of FJ11-1. This study represents the most comprehensive investigation of chromosome evolution and potential pathogenicity of mini-chromosomes in the genus.
炭疽病是由 物种引起的最常见的真菌病之一。这些症状通常表现为叶片、茎和果实上的深色、凹陷病变。在中国，芒果炭疽病严重影响果实的产量和质量。对几个物种的基因组测序表明存在小型染色体。这些被认为有助于毒力，但它们的形成和活性仍有待充分阐明。在这里，我们通过 PacBio 长读测序组装了 17 个基因组（16 个来自芒果，1 个来自柿子）。组装的支架中有一半的两端都有端粒重复序列，表明是完整的染色体。基于种间和种内的比较基因组分析，我们鉴定了广泛的染色体重排事件。我们分析了 spp.的小型染色体，发现近亲之间存在很大的变异。在 中，核心染色体和小型染色体之间的同源性表明，一些小型染色体是由核心染色体重组产生的。在 GZ23-3 中，我们发现 26 个水平转移基因排列在小型染色体上的簇中。在 FJ11-1 中，小型染色体上的几个潜在致病相关基因上调，特别是在高致病性表型的菌株中。这些上调基因的突变体在毒力方面表现出明显的缺陷。我们的研究结果为小型染色体与进化和潜在毒力的关系提供了新的见解。
是一个世界性的真菌属，严重影响许多植物物种的果实产量和质量。在 中发现小型染色体与毒力有关。进一步研究小型染色体可以帮助我们阐明 的一些致病机制。在这项研究中，我们生成了几个 菌株的新组装。在 种内和种间进行了比较基因组分析。然后我们系统地鉴定了我们测序菌株中的小型染色体。研究了小型染色体的特征和产生。转录组分析和基因敲除揭示了位于 FJ11-1 小型染色体上的与发病机制相关的基因。这项研究代表了对 属中小型染色体的染色体进化和潜在致病性的最全面研究。
