Institute of Plant Protection, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, 530007, China, and Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests, Nanning, Guangxi, 530007, China.
Department of Guangxi Forestry Pest Management, Nanning, Guangxi, 530028, China.
Plant Dis. 2018 Jul;102(7):1283-1289. doi: 10.1094/PDIS-09-17-1516-RE. Epub 2018 Apr 25.
Mango (Mangifera indica) is widely grown across southern China, especially in the provinces of Guangxi, Hainan, Yunnan, Sichuan, and Taiwan. Guangxi itself has over 86,667 ha of mango production. The purpose of this study was to identify Colletotrichum species associated with mango in different parts of Guangxi and examine their pathogenicity on leaves and fruits of mango in vitro. Diseased leaves were collected from 25 mango orchards in different areas of Guangxi province. Sixty-five isolates were obtained from mango leaves with anthracnose symptoms, and these were further characterized based on morphology and DNA sequencing. Twenty-nine isolates from different areas were selected for sequencing and analyses of the internal transcribed spacer region, glyceraldehyde-3-phosphate dehydrogenase, partial actin, β-tubulin, and chitin synthase genomic regions. The most common fungal isolates were these three species: Colletotrichum asianum, C. fructicola, and C. siamense. C. asianum was the most common and widely distributed in Guangxi (51.7%), followed by C. fructicola (37.9%) and C. siamense (10.2%), both found in Tiandong, Tianyang, and Wuming counties. There was no evidence of geographical specialization of the different species. Pathogenicity assays showed that all isolates were pathogenic to mango leaves and fruit (cultivar Tainong). No relationship was found between origin of isolates and their virulence. This is the first description of C. asianum, C. fructicola, and C. siamense as causal agents of mango leaf anthracnose from Guangxi province, China.
芒果（Mangifera indica）在中国南方广泛种植，尤其是在广西、海南、云南、四川和台湾等省份。广西本身的芒果种植面积就超过 86667 公顷。本研究的目的是鉴定与广西不同地区芒果相关的炭疽菌物种，并在体外检验它们对芒果叶片和果实的致病性。从广西不同地区的 25 个芒果园中采集了有炭疽病症状的叶片。从芒果叶片上采集了 65 个炭疽病的分离物，并根据形态和 DNA 测序对这些分离物进行了进一步的特征描述。从不同地区选择了 29 个分离物进行测序和分析，包括内部转录间隔区、甘油醛-3-磷酸脱氢酶、部分肌动蛋白、β-微管蛋白和几丁质合成酶基因组区域。最常见的真菌分离物是这三个物种：炭疽菌亚洲种、炭疽菌果实种和炭疽菌暹罗种。炭疽菌亚洲种最为常见，分布最广，在广西占 51.7%，其次是炭疽菌果实种（37.9%）和炭疽菌暹罗种（10.2%），这两种菌都在田东县、田阳县和武鸣县发现。没有证据表明不同物种具有地理专化性。致病性试验表明，所有分离物对芒果叶片和果实（田东 1 号品种）都具有致病性。分离物的起源与它们的毒性之间没有关系。这是首次在中国广西描述炭疽菌亚洲种、炭疽菌果实种和炭疽菌暹罗种是芒果叶炭疽病的病原菌。
