Lück Sarah, Westermark Pål O
Institute for Theoretical Biology, Charité - Universitätsmedizin Berlin, Invalidenstrasse 43, 10115, Berlin, Germany.
Cell Mol Life Sci. 2016 Feb;73(3):497-521. doi: 10.1007/s00018-015-2072-2. Epub 2015 Oct 26.
Circadian clocks synchronize organisms to the 24 h rhythms of the environment. These clocks persist under constant conditions, have their origin at the cellular level, and produce an output of rhythmic mRNA expression affecting thousands of transcripts in many mammalian cell types. Here, we review the charting of circadian output rhythms in mRNA expression, focusing on mammals. We emphasize the challenges in statistics, interpretation, and quantitative descriptions that such investigations have faced and continue to face, and outline remaining outstanding questions.
昼夜节律时钟使生物体与环境的24小时节律同步。这些时钟在恒定条件下持续存在,起源于细胞水平,并产生有节奏的mRNA表达输出,影响许多哺乳动物细胞类型中的数千种转录本。在这里,我们回顾了哺乳动物中mRNA表达的昼夜节律输出图谱。我们强调了此类研究已经面临并将继续面临的统计、解释和定量描述方面的挑战,并概述了仍然存在的突出问题。