Mwangi Joseph, Nganga Zipporah, Mpoke Solomon, Lihana Raphael, Kinyua Joyceline, Lagat Nancy, Muriuki Joseph, Lel Rency, Kageha Sheila, Osman Saida, Ichimura Hiroshi
Center for Virus Research, Kenya Medical Research Institute, P.O. Box 54628, Nairobi, Kenya.
Institute of Tropical Medicine and Infectious Disease, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya.
Arch Virol. 2016 Jan;161(1):95-101. doi: 10.1007/s00705-015-2623-8. Epub 2015 Oct 23.
Hepatitis C virus is a great public-health concern worldwide. Phylogenetic analysis of the HCV genome has identified six different genotypes that have generally been divided into several subtypes. There is very little information on HCV seroprevalence and genotypes in Kenya. To determine the genotypes of HCV circulating in Kenya, blood donor samples were serologically tested and confirmed by polymerase chain reaction (PCR). Positive samples were cloned and sequenced, and phylogenetic analysis conducted to determine the HCV genotypes. One hundred Murex-seropositive samples were re-tested using a passive hemagglutination test, and 16 of these were identified as seropositive. Further testing of all of the samples by PCR identified only 10 of the 16 samples as positive. Thus, only 10 % (10/100) of the samples were viremic. Six were from females (60 %), and four were from males (40 %). The mean age of the positive donors was considerably low, at 25 +/- 9 years. Genotypic testing indicated the presence of genotype 1a (10 %) and genotype 2b (90 %). This study reports on HCV genotypes in a blood donor population in Kenya where little had been done to provide information on HCV genotypes.
丙型肝炎病毒是全球重大的公共卫生问题。对丙肝病毒基因组的系统发育分析已确定了六种不同的基因型，这些基因型通常又被分为几个亚型。在肯尼亚，关于丙肝病毒血清流行率和基因型的信息非常少。为了确定在肯尼亚流行的丙肝病毒基因型，对献血者样本进行了血清学检测，并通过聚合酶链反应（PCR）进行了确认。对阳性样本进行克隆和测序，并进行系统发育分析以确定丙肝病毒基因型。使用被动血凝试验对100份默克血清阳性样本进行了重新检测，其中16份被确定为血清阳性。通过PCR对所有样本进行进一步检测，发现16份样本中只有10份为阳性。因此，只有10%（10/100）的样本存在病毒血症。其中6份来自女性（60%），4份来自男性（40%）。阳性献血者的平均年龄相当低，为25±9岁。基因型检测表明存在1a型（10%）和2b型（90%）。本研究报告了肯尼亚献血人群中的丙肝病毒基因型情况，此前在该方面几乎没有提供相关信息。
