Complex Systems Group, Department of Chemistry, University of Delhi, Delhi 110007, India.
Complex Systems Group, Department of Chemistry, University of Delhi, Delhi 110007, India.
Biosens Bioelectron. 2016 Mar 15;77:715-24. doi: 10.1016/j.bios.2015.10.039. Epub 2015 Oct 22.
Clinical manifestations owing to l-ascorbic acid for scurvy as comparison to d-ascorbic acid and challenges of chiral purity are overcome by using chiral selective conductive polymer nanocomposite which mimics antibodies and enzymes. A novel chiral selective imprinted polyaniline-ferrocene-sulfonic acid film has been electrochemically fabricated on C-dots modified pencil graphite electrode. The performance of the obtained l-ascorbic acid or d-ascorbic acid chiral selective sensor was investigated by electrochemical impedance spectroscopy, cyclic and differential pulse voltammetry. The surface characteristics of the C-dots, chiral sensor before and after the de-doping of chiral d- and l-ascorbic acid were characterized by scanning electron microscopy, Raman spectroscopy and X-ray diffraction spectroscopy. Excellent recognition results were obtained by difference in electron transfer resistance. The proposed chiral sensor is capable of measuring d-ascorbic acid or l-ascorbic acid in aqueous as well as in real and commercial samples within the range of 0.020-0.187 nM and 0.003-0.232 nM with detection limit of 0.00073 nM and 0.00016 nM, respectively. The proposed method has also been examined for the chiral selective recognition of ascorbic acid isomers (d- and l-) quantitatively, in complicated matrices of real samples.
由于抗坏血酸引起的临床症状与 d-抗坏血酸相比，手性纯度的挑战通过使用模拟抗体和酶的手性选择导电聚合物纳米复合材料得以克服。一种新型的手性选择印迹聚苯胺-二茂铁-磺酸膜已在 C 点修饰的铅笔石墨电极上通过电化学方法制备。通过电化学阻抗谱、循环伏安法和差分脉冲伏安法研究了所得 l-抗坏血酸或 d-抗坏血酸手性选择传感器的性能。通过电子转移电阻的差异获得了出色的识别结果。所提出的手性传感器能够在水相以及真实和商业样品中测量 d-抗坏血酸或 l-抗坏血酸，范围分别为 0.020-0.187 nM 和 0.003-0.232 nM，检测限分别为 0.00073 nM 和 0.00016 nM。该方法还用于对手性选择识别抗坏血酸异构体（d-和 l-）进行定量分析，包括真实样品的复杂基质。
