Fukumitsu Satoshi, Villareal Myra O, Fujitsuka Takashi, Aida Kazuhiko, Isoda Hiroko
Nippon Flour Mills Co, Ltd, Central Laboratory, Midorigaoka, Atsugi, Kanagawa, Japan.
Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
Mol Nutr Food Res. 2016 Feb;60(2):399-409. doi: 10.1002/mnfr.201500465. Epub 2015 Nov 17.
Consumption of olives (Olea europaea L.), including table olives and oil, is associated with low incidence of inflammation-related diseases. In this study, the effects of maslinic acid (MA), the main constituent of olive pomace, on the expression of genes and proteins involved in inflammatory activity in RAW 264.7 cells were investigated. Furthermore, the effect of MA on carrageenan-induced paw edema and collagen antibody induced arthritis in mice was determined.
We confirmed the suppressive effects of MA on LPS-induced tumor necrosis factor α production and on the expression of inflammatory response associated genes in RAW 264.7 cells. We also clarified the suppressive effect of MA on LPS-induced nuclear factor-kappa B activation as well as the phosphorylation of IκB-α. Furthermore, MA (200 mg/kg in the edema model or 100 mg/kg in the arthritis model) exerted anti-inflammatory and antiarthritis effects as shown by the suppression of paw edema, arthritis score, inflammatory cells, and destruction of synovium in knee joints.
Olive products containing MA are useful as a new preventive and therapeutic food ingredient for inflammatory and arthritic diseases.
食用橄榄（油橄榄），包括食用橄榄果和橄榄油，与炎症相关疾病的低发病率有关。在本研究中，研究了橄榄果渣的主要成分山楂酸（MA）对RAW 264.7细胞中炎症活性相关基因和蛋白质表达的影响。此外，还确定了MA对小鼠角叉菜胶诱导的爪肿胀和胶原抗体诱导的关节炎的作用。
我们证实了MA对RAW 264.7细胞中脂多糖（LPS）诱导的肿瘤坏死因子α产生以及炎症反应相关基因表达的抑制作用。我们还阐明了MA对LPS诱导的核因子κB激活以及IκB-α磷酸化的抑制作用。此外，MA（在肿胀模型中为200mg/kg，在关节炎模型中为100mg/kg）通过抑制爪肿胀、关节炎评分、炎症细胞以及膝关节滑膜破坏，发挥了抗炎和抗关节炎作用。
含有MA的橄榄产品可作为炎症性和关节炎性疾病的新型预防和治疗食品成分。
