Wang Kun, Tang Rong-Yu, Zhao Xiao-Bo, Li Jun-Jie, Lang Yi-Ran, Jiang Xiao-Xia, Sun Hong-Ji, Lin Qiu-Xia, Wang Chang-Yong
Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical Sciences, No. 27, Taiping Road, Beijing, 100850, China.
Nanoscale. 2015 Nov 28;7(44):18677-85. doi: 10.1039/c5nr05784a. Epub 2015 Oct 26.
The development of coating materials for neural interfaces has been a pursued to improve the electrical, mechanical and biological performances. For these goals, a bioactive coating was developed in this work featuring a poly(3,4-ethylenedioxythiophene) (PEDOT)/carbon nanotube (CNT) composite and covalently bonded YIGSR and RGD. Its biological effect and electrical characteristics were assessed in vivo on microwire arrays (MWA). The coated electrodes exhibited a significantly higher charge storage capacity (CSC) and lower electrochemical impedance at 1 kHz which are desired to improve the stimulating and recording performances, respectively. Acute neural recording experiments revealed that coated MWA possess a higher signal/noise ratio capturing spikes undetected by uncoated electrodes. Moreover, coated MWA possessed more active sites and single units, and the noise floor of coated electrodes was lower than that of uncoated electrodes. There is little information in the literature concerning the chronic performance of bioactively modified neural interfaces in vivo. Therefore in this work, chronic in vivo tests were conducted and the PEDOT/PSS/MWCNT-polypeptide coated arrays exhibited excellent performances with the highest mean maximal amplitude from day 4 to day 12 during which the acute response severely compromised the performance of the electrodes. In brief, we developed a simple method of covalently bonding YIGSR and RGD to a PEDOT/PSS/MWCNT-COOH composite improving both the biocompatibility and electrical performance of the neural interface. Our findings suggest that YIGSR and RGD modified PEDOT/PSS/MWCNT is a promising bioactivated composite coating for neural recording and stimulating.
开发用于神经接口的涂层材料一直是为了改善其电学、机械和生物学性能。为了实现这些目标，本研究开发了一种具有生物活性的涂层，其特征在于聚（3,4-乙撑二氧噻吩）（PEDOT）/碳纳米管（CNT）复合材料以及共价结合的YIGSR和RGD。在体内对微线阵列（MWA）评估了其生物学效应和电学特性。涂覆的电极在1 kHz时表现出显著更高的电荷存储容量（CSC）和更低的电化学阻抗，分别有利于改善刺激和记录性能。急性神经记录实验表明，涂覆的MWA具有更高的信噪比，能够捕获未涂覆电极未检测到的尖峰信号。此外，涂覆的MWA具有更多的活性位点和单个单元，并且涂覆电极的本底噪声低于未涂覆电极。关于体内生物活性修饰神经接口的长期性能，文献中几乎没有相关信息。因此，在本研究中进行了长期体内测试，PEDOT/PSS/MWCNT-多肽涂覆的阵列表现出优异的性能，在第4天至第12天期间平均最大振幅最高，在此期间急性反应严重损害了电极的性能。简而言之，我们开发了一种将YIGSR和RGD共价结合到PEDOT/PSS/MWCNT-COOH复合材料上的简单方法，改善了神经接口的生物相容性和电学性能。我们的研究结果表明，YIGSR和RGD修饰的PEDOT/PSS/MWCNT是一种用于神经记录和刺激的有前景的生物活性复合涂层。
