Institute of Chemistry, State University of Campinas (UNICAMP), P.O. Box 6154, 13083-970 Campinas, SP, Brazil; National Institute of Science and Technology of Bioanalytics (INCTBio), Institute of Chemistry - UNICAMP, P.O. Box 6154, 13084-971 Campinas, SP, Brazil.
Programa de Pós-graduação em Biotecnologia Industrial - Universidade Tiradentes, Aracaju, SE, Brazil.
Biosens Bioelectron. 2016 Mar 15;77:746-51. doi: 10.1016/j.bios.2015.10.042. Epub 2015 Oct 23.
A hybrid platform based on ionic liquid-based alkoxysilane functionalized mesoporous silica was applied for the synthesis of supported Pt nanoparticles with peroxidase-like catalytic activity. The positively charged groups (imidazolium) chemically bonded to the surface provide dual-functionality as ion-exchangers to the hybrid material, firstly used for the in situ synthesis of the highly dispersed Pt nanostructures and, secondly, for the immobilization of biological species aiming biosensing purposes. The peroxidase-like catalytic activity of the SiO2/Imi/Pt material was evaluated towards the H2O2-mediated oxidation of a chromogenic peroxidase substrate (TMB), allowing the colorimetric detection of H2O2. Finally, to further explore the practical application of this nanomaterial-based artificial system, glucose oxidase (GOx) was immobilized on the catalytic porous platform and a bioassay for the colorimetric determination of glucose was successfully conducted as a model system. The enzyme-like catalytic properties of the SiO2/Imi/Pt as well as its ability to immobilize and keep active biological entities on the porous structure indicate that this hybrid porous platform is potentially useful for the development of biosensing devices.
一种基于离子液体修饰的烷氧基硅烷的杂化平台被应用于合成具有过氧化物酶样催化活性的负载型 Pt 纳米粒子。带正电荷的基团（咪唑鎓）与表面化学结合，为杂化材料提供了双重功能，首先用于高度分散的 Pt 纳米结构的原位合成，其次用于生物物种的固定化，以实现生物传感目的。SiO2/Imi/Pt 材料的过氧化物酶样催化活性通过 H2O2 介导的显色过氧化物酶底物（TMB）的氧化进行评估，允许对 H2O2 进行比色检测。最后，为了进一步探索基于这种纳米材料的人工系统的实际应用，将葡萄糖氧化酶（GOx）固定在催化多孔平台上，并成功地进行了葡萄糖的比色测定生物测定作为模型系统。SiO2/Imi/Pt 的酶样催化性质以及其在多孔结构上固定和保持生物实体活性的能力表明，这种杂化多孔平台对于开发生物传感设备具有潜在的用途。
