School of Materials Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China; Department of Chemistry, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai 200433, China.
Department of Chemistry, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai 200433, China.
J Colloid Interface Sci. 2020 Jun 15;570:300-311. doi: 10.1016/j.jcis.2020.02.118. Epub 2020 Mar 3.
In past decade, Pt-based nanomaterials as peroxidase mimics have attracted much attention for HO and glucose detection. However, easy aggregation of Pt nanoparticles (Pt NPs) greatly decreases their peroxidase-like activity. In this work, novel Pt/EMT nanocomposites were prepared by uniformly loading Pt NPs (5-8 nm) onto the support of ultrasmall EMT zeolite (15-20 nm), a kind of low-silica microporous aluminosilicate material. The hybrid Pt/EMT nanomaterials could be well dispersed in water to form a homogeneous suspension, and were then utilized as a superior peroxidase-like catalyst for oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide (HO). The optimal catalyst of 2.6Pt/EMT nanocomposite exhibited excellent catalytic performance toward HO and TMB than natural enzyme of horseradish peroxidase (HRP) by using a steady-state kinetic analysis based on the typical Michaelis-Menten kinetics theory. The peroxidase-like catalyst showed a promising activity in a wide pH and temperature range as well as the long-term stability. A facile and reliable colorimetric assay based on the peroxidase mimic of Pt/EMT nanocomposite was constructed for precise detection of HO and glucose in a wide linear range, with low limits of detection of 1.1 μM and 13.2 μM, respectively. Due to high selectivity to glucose against other sugars on the catalyst, the method was demonstrated to accurately measure the concentration of glucose in real samples including human blood serum and fruit juices, indicating a potential application of the Pt/EMT nanocomposites as a robust peroxidase mimic and a reliable biosensor in the fields of clinical diagnosis, pharmaceutical, food research and so on.
在过去的十年中，基于铂的纳米材料作为过氧化物酶模拟物，因其在 HO 和葡萄糖检测方面的应用而受到广泛关注。然而，铂纳米颗粒（Pt NPs）容易聚集，极大地降低了它们的过氧化物酶样活性。在这项工作中，通过将 5-8nm 的 Pt NPs 均匀负载到超小 EMT 沸石（15-20nm）的载体上，制备了新型 Pt/EMT 纳米复合材料。这种低硅微孔铝硅酸盐材料。混合的 Pt/EMT 纳米材料可以很好地分散在水中，形成均匀的悬浮液，然后在过氧化氢（HO）存在下用作 TMB 氧化的优异过氧化物酶样催化剂。通过基于典型米氏动力学理论的稳态动力学分析，发现 2.6Pt/EMT 纳米复合材料的最佳催化剂对 HO 和 TMB 的催化性能优于辣根过氧化物酶（HRP）的天然酶。该过氧化物酶模拟物在宽 pH 和温度范围以及长期稳定性方面表现出良好的活性。基于 Pt/EMT 纳米复合材料的过氧化物酶模拟物，构建了一种简单可靠的比色测定法，用于 HO 和葡萄糖的精确检测，具有低检测限分别为 1.1μM 和 13.2μM。由于该催化剂对葡萄糖具有较高的选择性，该方法可用于准确测量包括人血清和果汁在内的实际样品中的葡萄糖浓度，表明 Pt/EMT 纳米复合材料作为一种稳健的过氧化物酶模拟物和可靠的生物传感器，在临床诊断、制药、食品研究等领域具有潜在的应用前景。
