Mishra Debasish, Gopalakrishnan Sivaram, Arun K V, Kumar Tirunveli Saravanan Subu, Devanathan Santosh, Misra Satya Ranjan
Senior Resident, Department of Periodontics, SCB Dental College & Hospital , Cuttack, Odisha, India .
Professor, Department of Periodontics, Ragas Dental College & Hospital , Chennai, Tamil Nadu, India .
J Clin Diagn Res. 2015 Sep;9(9):ZC50-5. doi: 10.7860/JCDR/2015/12689.6498. Epub 2015 Sep 1.
Traditional parameters (Pocket depth, bleeding on probing, clinical attachment loss, radiographic findings) have been used for a long time for the assessment of periodontal disease conditions. However, these parameters only indicate towards the periodontal damage that has already taken place but do not give any idea regarding the current status of the periodontal health or disease. Hence, the present study is aimed at evaluating the concentration of the bone biomarker ICTP in saliva, which can give a better real time assessment of periodontal health and disease.
Forty three patients were selected and divided into three groups based on the recorded clinical parameters of probing pocket depth, attachment loss and bleeding on probing. Group I (Healthy, n = 11), Group II (Gingivitis, n = 17), Group III (Periodontitis. n = 15). Salivary samples were collected before scaling and root planning to avoid contamination by blood. ICTP levels were evaluated in the salivary samples by using enzyme linked immunosorbent assay (ELISA).
Kruskal Wallis test was used to compare the mean ICTP level of the three groups.
ICTP was detected in all the samples. Highest mean ICTP concentrations in saliva were obtained for group III (periodontitis group) and the lowest mean ICTP concentrations were seen in group I (healthy group). This suggests that the level of ICTP in saliva increases proportionally from periodontal health to diseased conditions (gingivitis & periodontitis).
There is a substantial increase in the salivary concentration of ICTP in chronic periodontitis patients than in gingivitis and healthy patients. Salivary ICTP levels were the maximum in chronic periodontitis patients followed by gingivitis patients and the least in healthy individuals. ICTP may be considered as a biomarker in periodontal disease progression.
传统参数（牙周袋深度、探诊出血、临床附着丧失、影像学表现）长期以来一直用于评估牙周疾病状况。然而，这些参数仅表明已经发生的牙周损伤，对于牙周健康或疾病的当前状态却没有任何提示。因此，本研究旨在评估唾液中骨生物标志物Ⅰ型胶原羧基端肽（ICTP）的浓度，其能够更好地实时评估牙周健康和疾病状况。
选取43例患者，根据记录的探诊牙周袋深度、附着丧失和探诊出血等临床参数分为三组。第一组（健康组，n = 11），第二组（牙龈炎组，n = 17），第三组（牙周炎组，n = 15）。在进行龈下刮治和根面平整之前采集唾液样本，以避免血液污染。采用酶联免疫吸附测定（ELISA）法评估唾液样本中的ICTP水平。
采用Kruskal Wallis检验比较三组的平均ICTP水平。
所有样本中均检测到ICTP。第三组（牙周炎组）唾液中的平均ICTP浓度最高，第一组（健康组）的平均ICTP浓度最低。这表明唾液中ICTP的水平从牙周健康状态到患病状态（牙龈炎和牙周炎）呈比例增加。
慢性牙周炎患者唾液中ICTP的浓度比牙龈炎患者和健康患者显著升高。慢性牙周炎患者的唾液ICTP水平最高，其次是牙龈炎患者，健康个体中最低。ICTP可被视为牙周疾病进展的生物标志物。
