Osho Adeleke, Otuechere Chiagoziem A, Adeosun Charles B, Oluwagbemi Tolu, Atolani Olubunmi
J Basic Clin Physiol Pharmacol. 2016 Mar;27(2):163-70. doi: 10.1515/jbcpp-2015-0031.
In Nigeria, Cordia sebestena (Boraginaceae), an understudied medicinal plant, is used in traditional medicine for the treatment of gastrointestinal disorders. In this study, we investigated the chemical composition, antibacterial potential, and sub-acute toxicity of C. sebestena leaves.
Ethyl acetate extracts were analyzed using thin layer chromatography (TLC) and Fourier transform infrared (FTIR) spectrophotometry. The antibacterial potential of the extracts was tested against five standard bacteria, namely Bacillus cereus, Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Clinical observations and blood parameters were used to evaluate the possible toxicity of C. sebestena.
The TLC profile yielded 39 fractions, which were pooled to nine combined sub-fractions (A-I). The FTIR spectrum of sub-fraction H indicated the presence of aliphatic C-H stretching vibration at 2922 and 2850 cm-1, C=O stretch at 1734 and 1708 cm-1, and C=C stretch of aromatics and aliphatics at 1464 and (shoulder) 1618 cm-1, respectively. The fractions of the C. sebestena ethyl acetate leaf extract showed antibacterial potential across board, but fraction H had the highest antibacterial activity against B. cereus and S. aureus. The study also indicated the relatively low toxicity profile of the ethyl acetate leaf extract of C. sebestena in the liver of rats.
The study showed that C. sebestena leaves have strong antibacterial potential and low toxicity, thereby underlying the scientific basis for their folkloric use in the management of microbial infections and its associated complications.
在尼日利亚，塞贝斯特破布木（紫草科）是一种研究较少的药用植物，在传统医学中用于治疗胃肠道疾病。在本研究中，我们调查了塞贝斯特破布木叶的化学成分、抗菌潜力和亚急性毒性。
使用薄层色谱法（TLC）和傅里叶变换红外（FTIR）分光光度法分析乙酸乙酯提取物。测试提取物对五种标准细菌的抗菌潜力，这五种细菌分别为蜡样芽孢杆菌、枯草芽孢杆菌、金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌。通过临床观察和血液参数评估塞贝斯特破布木可能的毒性。
TLC图谱产生了39个馏分，这些馏分被合并为9个组合子馏分（A-I）。子馏分H的FTIR光谱表明，在2922和2850 cm-1处存在脂肪族C-H伸缩振动，在1734和1708 cm-1处存在C=O伸缩振动，在1464和（肩峰）1618 cm-1处分别存在芳香族和脂肪族的C=C伸缩振动。塞贝斯特破布木乙酸乙酯叶提取物的馏分均显示出抗菌潜力，但馏分H对蜡样芽孢杆菌和金黄色葡萄球菌具有最高的抗菌活性。该研究还表明，塞贝斯特破布木乙酸乙酯叶提取物在大鼠肝脏中的毒性相对较低。
该研究表明，塞贝斯特破布木叶具有很强的抗菌潜力且毒性较低，从而为其在民间用于治疗微生物感染及其相关并发症提供了科学依据。
