Poellmann Michael J, Estrada Jonathan B, Boudou Thomas, Berent Zachary T, Franck Christian, Wagoner Johnson Amy J
J Biomech Eng. 2015 Dec;137(12):124503. doi: 10.1115/1.4031848.
Osteogenesis is the process by which mesenchymal stem cells differentiate to osteoblasts and form bone. The morphology and root mean squared (RMS) traction of four cell types representing different stages of osteogenesis were quantified. Undifferentiated D1, differentiated D1, MC3T3-E1, and MLO-A5 cell types were evaluated using both automated image analysis of cells stained for F-actin and by traction force microscopy (TFM). Undifferentiated mesenchymal stem cell lines were small, spindly, and exerted low traction, while differentiated osteoblasts were large, had multiple processes, and exerted higher traction. Size, shape, and traction all correlated with the differentiation stage. Thus, cell morphology evolved and RMS traction increased with differentiation. The results provide a foundation for further work with these cell lines to study the mechanobiology of bone formation.
骨生成是间充质干细胞分化为成骨细胞并形成骨的过程。对代表骨生成不同阶段的四种细胞类型的形态和均方根（RMS）牵引力进行了量化。使用针对F-肌动蛋白染色的细胞自动图像分析和牵引力显微镜（TFM）对未分化的D1、分化的D1、MC3T3-E1和MLO-A5细胞类型进行了评估。未分化的间充质干细胞系体积小、呈纺锤形且牵引力低，而分化的成骨细胞体积大、有多个突起且牵引力更高。大小、形状和牵引力均与分化阶段相关。因此，细胞形态随着分化而演变，RMS牵引力增加。这些结果为进一步利用这些细胞系研究骨形成的力学生物学奠定了基础。
