Mei Z Q, Fu S Y, Yu H Q, Yang L Q, Duan C G, Liu X Y, Gong S, Fu J J
Research Center for Preclinical Medicine, Luzhou Medical College, Luzhou, Sichuan Province, China.
Michael E. DeBakey High School for Health Professions, Houston, TX, USA.
Genet Mol Res. 2014 Mar 6;13(1):1447-55. doi: 10.4238/2014.March.6.3.
Dimocarpus longan Lour. is an edible and traditional herb in China, commonly referred to as longon. An improved randomly amplified polymorphic DNA (RAPD) protocol was here developed in order to determine the geographical origins of D. longan samples collected from 5 provinces in the southern and southwestern areas of China, including Sichuan, Hainan, Fujian, Guangdong, and Guangxi. Generally, the improved RAPD method generated good fingerprinting of the 5 samples using the selected 17 primers. In particular, primers SBS-A5, SBS-A13, SBS-I9, SBS-I20, SBS-M1, and SBS-Q12 produced distinguishable bands that clearly separated all 5 cultivars, suggesting that there are variations in RAPD genetic sites among the samples. The similarity index ranged from 0.69 to 0.76. The Sichuan and Hainan clades clustered together with a 0.73 similarity index. The Guangxi and Fujian clades clustered together with a 0.76 similarity index, and they formed the sister clade to the Sichuan/Hainan clade with a 0.71 similarity index. The Guangdong clade was in a basal polytomy with a 0.70 similarity index. Based on the abundant DNA polymorphisms, these longan accessions are distinguishable using our improved RAPD technique. Therefore, RAPD analysis is an effective technique in distinguishing the geographical origins of D. longan. Moreover, the improved method could also be employed for a variety of applications including genetic diversity and fingerprinting analyses.
龙眼是中国一种可食用的传统草药，通常被称为桂圆。为了确定从中国南部和西南部5个省份（包括四川、海南、福建、广东和广西）采集的龙眼样本的地理来源，在此开发了一种改进的随机扩增多态性DNA（RAPD）方案。一般来说，改进的RAPD方法使用选定的17个引物对5个样本产生了良好的指纹图谱。特别是，引物SBS - A5、SBS - A13、SBS - I9、SBS - I20、SBS - M1和SBS - Q12产生了可区分的条带，清楚地将所有5个品种区分开来，表明样本之间的RAPD基因位点存在差异。相似性指数范围为0.69至0.76。四川和海南分支以0.73的相似性指数聚类在一起。广西和福建分支以0.76的相似性指数聚类在一起，并且它们以0.71的相似性指数形成了四川/海南分支的姐妹分支。广东分支处于基部多歧分支，相似性指数为0.70。基于丰富的DNA多态性，使用我们改进的RAPD技术可以区分这些龙眼种质。因此，RAPD分析是区分龙眼地理来源的有效技术。此外，改进后的方法还可用于包括遗传多样性和指纹图谱分析在内的各种应用。
