为欧洲鹅耳枥（桦木科）开发的微卫星标记的特征及可转移性(1)
Characterization and transferability of microsatellite markers developed for Carpinus betulus (Betulaceae)(1).
作者信息
Prinz Kathleen, Finkeldey Reiner
机构信息
Institute for Systematic Botany with Herbarium Haussknecht and Botanical Garden, Friedrich-Schiller-Universität Jena, Jena, Germany ; Section Forest Genetics and Forest Tree Breeding, Georg-August-Universität Göttingen, Göttingen, Germany.
Section Forest Genetics and Forest Tree Breeding, Georg-August-Universität Göttingen, Göttingen, Germany.
出版信息
Appl Plant Sci. 2015 Oct 12;3(10). doi: 10.3732/apps.1500053. eCollection 2015 Oct.
PREMISE OF THE STUDY
Carpinus betulus (Betulaceae) is an octoploid, ecologically important, common tree species in European woodlands. We established 11 nuclear microsatellite loci allowing for detailed analyses of genetic diversity and structure.
METHODS AND RESULTS
A microsatellite-enriched library was used to develop primers for 11 microsatellite loci that revealed high allele numbers and genetic diversity in a preliminary study.
CONCLUSIONS
All of the loci developed here are informative for C. betulus. In addition, the loci are transferable to several species within the genus, and almost all loci cross-amplified in species of different genera of the Betulaceae.
研究前提
欧洲鹅耳枥（桦木科）是一种八倍体、具有重要生态意义的常见树种，生长于欧洲林地。我们建立了11个核微卫星位点，以便对遗传多样性和结构进行详细分析。
方法与结果
利用一个微卫星富集文库开发了11个微卫星位点的引物，在初步研究中显示出高等位基因数和遗传多样性。
结论
这里开发的所有位点对欧洲鹅耳枥都具有信息价值。此外，这些位点可转移到该属的几个物种，并且几乎所有位点在桦木科不同属的物种中都能交叉扩增。
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