Sheng Qianqian, Fang Xianying, Zhu Zunling, Xiao Wei, Wang Zhenzhong, Ding Gang, Zhao Linguo, Li Yujian, Yu Ping, Ding Zhibin, Sun Qinru
College of Landscape Architecture, Nanjing Forestry University, Nanjing, 210037, China.
College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, China.
In Vitro Cell Dev Biol Anim. 2016 Jun;52(6):654-61. doi: 10.1007/s11626-016-0041-1. Epub 2016 Apr 25.
The genus Carpinus of Betulaceae is the most widely distributed in the European landscape. This study reports a comparative study based on the pheophorbide a and flavonoid content from the two main species of the genus Carpinus, Carpinus betulus and Carpinus turczaninowii, respectively, in Nanjing, China. The pheophorbide a and flavonoid content depends on the organ, species, and season. HPLC analysis showed that the pheophorbide a and flavonoid levels were the highest in May and June, respectively, from the leaves of C. betulus 'Fastigiata.' In contrast, the content of pheophorbide a and flavonoid in the stems of C. betulus 'Fastigiata' or in other species was low. The immunosuppressive effects of the ethyl acetate extracts and methanol extracts from the two Carpinus species were also evaluated. The ethyl acetate extracts of C. betulus 'Fastigiata' in May and the methanol extracts of C. betulus 'Fastigiata' in June showed better immunosuppressive activity than in other seasons, which coincided with the content of pheophorbide a and flavonoid, respectively. Our findings indicated that C. betulus 'Fastigiata' can serve as a medicinal plant against inflammation because of its pheophorbide a and flavonoid content.
桦木科鹅耳枥属植物在欧洲景观中分布最为广泛。本研究报告了一项基于中国南京鹅耳枥属两个主要物种——欧洲鹅耳枥和鹅耳枥的脱镁叶绿酸a及黄酮类化合物含量的比较研究。脱镁叶绿酸a和黄酮类化合物的含量取决于器官、物种和季节。高效液相色谱分析表明，欧洲鹅耳枥‘塔形’叶片中的脱镁叶绿酸a和黄酮类化合物含量分别在5月和6月最高。相比之下，欧洲鹅耳枥‘塔形’茎部或其他物种中的脱镁叶绿酸a和黄酮类化合物含量较低。还评估了这两种鹅耳枥属植物的乙酸乙酯提取物和甲醇提取物的免疫抑制作用。5月欧洲鹅耳枥‘塔形’的乙酸乙酯提取物和6月欧洲鹅耳枥‘塔形’的甲醇提取物分别比其他季节表现出更好的免疫抑制活性，这分别与脱镁叶绿酸a和黄酮类化合物的含量一致。我们的研究结果表明，欧洲鹅耳枥‘塔形’因其脱镁叶绿酸a和黄酮类化合物含量可作为一种抗炎药用植物。
