Mamede A C, Guerra S, Laranjo M, Carvalho M J, Oliveira R C, Gonçalves A C, Alves R, Prado Castro L, Sarmento-Ribeiro A B, Moura P, Abrantes A M, Maia C J, Botelho M F
Biophysics Unit, Faculty of Medicine, University of Coimbra, Azinhaga de Santa Comba - Celas, 3000-548, Coimbra, Portugal.
CICS-UBI, Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal.
Med Oncol. 2015 Dec;32(12):257. doi: 10.1007/s12032-015-0702-z. Epub 2015 Oct 27.
Hepatocellular carcinoma (HCC) has a worldwide high incidence and mortality. For this reason, it is essential to invest in new therapies for this type of cancer. Our team already proved that human amniotic membrane (hAM) is able to inhibit the metabolic activity of several human cancer cell lines, including HCC cell lines. Taking into account the previously performed work, this experimental study aimed to investigate the pathways by which hAM protein extracts (hAMPEs) act on HCC. Our results showed that hAMPE reduce the metabolic activity, protein content and DNA content in a dose- and time-dependent manner in all HCC cell lines. This therapy presents selective cytotoxicity, since it was not able to inhibit a non-tumorigenic human cell line. In addition, hAMPE induced cell morphology alterations in all HCC cell lines, but death type is cell line dependent, as proved by in vitro and in vivo studies. In conclusion, hAMPE have a promising role in HCC therapy, since it is capable of inducing HCC cytotoxicity and cell death.
肝细胞癌（HCC）在全球范围内具有较高的发病率和死亡率。因此，投资研发针对此类癌症的新疗法至关重要。我们的团队已经证明，人羊膜（hAM）能够抑制包括肝癌细胞系在内的多种人类癌细胞系的代谢活性。考虑到之前所开展的工作，本实验研究旨在探究hAM蛋白提取物（hAMPEs）作用于肝癌的途径。我们的结果表明，hAMPEs在所有肝癌细胞系中均以剂量和时间依赖性方式降低代谢活性、蛋白质含量和DNA含量。这种疗法具有选择性细胞毒性，因为它无法抑制非致瘤性人类细胞系。此外，hAMPEs在所有肝癌细胞系中均诱导细胞形态改变，但细胞死亡类型取决于细胞系，体外和体内研究均已证明这一点。总之，hAMPEs在肝癌治疗中具有广阔前景，因为它能够诱导肝癌细胞产生细胞毒性并导致细胞死亡。
