Mamede A C, Guerra S, Laranjo M, Santos K, Carvalho M J, Carvalheiro T, Moura P, Paiva A, Abrantes A M, Maia C J, Botelho M F
Biophysics Unit, Faculty of Medicine, University of Coimbra, Azinhaga de Santa Comba - Celas, 3000-548, Coimbra, Portugal.
CICS-UBI, Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal.
Pathol Oncol Res. 2016 Oct;22(4):689-97. doi: 10.1007/s12253-016-0053-x. Epub 2016 Mar 10.
The anticancer effects of human amniotic membrane (hAM) have been studied over the last decade. However, the action mechanisms responsible for these effects are not fully understood until now. Previously results reported by our team proved that hAM is able to induce cytotoxicity and cell death in hepatocellular carcinoma (HCC), a worldwide high incident and mortal cancer. Therefore, this experimental study aimed to investigate the cellular targets of hAM protein extracts (hAMPE) in HCC through in vitro studies. Our results showed that hAMPE is able to modify oxidative stress environment in all HCC cell lines, as well as its cell cycle. hAMPE differently targets deoxyribonucleic acid (DNA), P21, P53, β-catenin and multidrug resistance (MDR) proteins in HCC cell lines. In conclusion, hAMPE has several targets in HCC, being clear that the success of this treatment depends of a personalized therapy based on the biological and genetic characteristics of the tumor.
在过去十年中，人们对人羊膜（hAM）的抗癌作用进行了研究。然而，迄今为止，导致这些作用的作用机制尚未完全明确。此前我们团队报道的结果证明，hAM能够在肝细胞癌（HCC）中诱导细胞毒性和细胞死亡，肝细胞癌是一种在全球范围内具有高发病率和高死亡率的癌症。因此，本实验研究旨在通过体外研究来探究hAM蛋白提取物（hAMPE）在肝癌中的细胞靶点。我们的结果表明，hAMPE能够改变所有肝癌细胞系中的氧化应激环境及其细胞周期。hAMPE在肝癌细胞系中对脱氧核糖核酸（DNA）、P21、P53、β-连环蛋白和多药耐药（MDR）蛋白具有不同的靶向作用。总之，hAMPE在肝癌中有多个靶点，很明显，这种治疗方法的成功取决于基于肿瘤生物学和遗传学特征的个性化治疗。
