Barskiĭ V E, Egorov E E, Kreĭndlin É Ia, Lysov Iu P, Pan'kov S V, Iurasov D A, Iurasov R A, Zasedatelev A S
Biofizika. 2012 May-Jun;57(3):522-7.
This paper discusses an issue on the development of biophysical methods for biochip analysis. A scheme and construction of a biochip analyzer based on wide-field digital fluorescence microscopy are described. The analyzer is designed to register images of biological microchips labeled with fluorescent dyes. The device developed is useful for high-sensitive throughput recording analyses by biochips after interaction of immobilized probes with fluorescently labeled sample molecules as well as it provides the higher rate of the analysis compared to laser scanning devices. With this analyzer a scope where biological microchips can be applied becomes wider, the development of new protocols of the analyses is possible and standard analyses run faster with the use of biochips, the expenses for the analysis performance can be reduced.
本文讨论了生物芯片分析生物物理方法发展中的一个问题。描述了基于宽场数字荧光显微镜的生物芯片分析仪的方案和构造。该分析仪旨在记录用荧光染料标记的生物微芯片的图像。所开发的设备对于固定化探针与荧光标记的样品分子相互作用后生物芯片的高灵敏度通量记录分析很有用，并且与激光扫描设备相比，它提供了更高的分析速率。使用该分析仪，生物微芯片的应用范围变得更广，可以开发新的分析方案，使用生物芯片进行标准分析运行得更快，分析性能的费用可以降低。
