Chen Chuck T, Kitson Alex P, Hopperton Kathryn E, Domenichiello Anthony F, Trépanier Marc-Olivier, Lin Lauren E, Ermini Leonardo, Post Martin, Thies Frank, Bazinet Richard P
Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, FitzGerald Building, 150 College St., Room 306, Toronto, ON, Canada, M5S 3E2.
Program in Physiology and Experimental Medicine, Peter Gilgan Centre for Research and Learning, The Hospital for Sick Children, Toronto, ON, Canada, M5G 0A4.
Sci Rep. 2015 Oct 29;5:15791. doi: 10.1038/srep15791.
Despite being critical for normal brain function, the pools that supply docosahexaenoic acid (DHA) to the brain are not agreed upon. Using multiple kinetic models in free-living adult rats, we first demonstrate that DHA uptake from the plasma non-esterified fatty acid (NEFA) pool predicts brain uptake of DHA upon oral administration, which enters the plasma NEFA pool as well as multiple plasma esterified pools. The rate of DHA loss by the brain is similar to the uptake from the plasma NEFA pool. Furthermore, upon acute iv administration, although more radiolabeled lysophosphatidylcholine (LPC)-DHA enters the brain than NEFA-DHA, this is due to the longer plasma half-life and exposure to the brain. Direct comparison of the uptake rate of LPC-DHA and NEFA-DHA demonstrates that uptake of NEFA-DHA into the brain is 10-fold greater than LPC-DHA. In conclusion, plasma NEFA-DHA is the major plasma pool supplying the brain.
尽管为大脑正常功能提供二十二碳六烯酸（DHA）的来源尚未达成共识，但它对大脑正常功能至关重要。我们使用多个动力学模型对成年自由活动大鼠进行研究，首先证明口服给药后，从血浆非酯化脂肪酸（NEFA）池中摄取的DHA可预测大脑对DHA的摄取情况，口服的DHA会进入血浆NEFA池以及多个血浆酯化池。大脑中DHA的流失速率与从血浆NEFA池中的摄取速率相似。此外，急性静脉注射时，虽然进入大脑的放射性标记溶血磷脂酰胆碱（LPC）-DHA比NEFA-DHA多，但这是由于LPC-DHA的血浆半衰期更长且在大脑中的暴露时间更长。对LPC-DHA和NEFA-DHA摄取速率的直接比较表明，NEFA-DHA进入大脑的摄取量比LPC-DHA大10倍。总之，血浆NEFA-DHA是为大脑提供DHA的主要血浆来源。
