Karlsson Patricia, Christie Michael Danger, Seymour Danelle K, Wang Huan, Wang Xi, Hagmann Jörg, Kulcheski Franceli, Manavella Pablo Andrés
Max Planck Institute for Developmental Biology, D-72076 Tübingen, Germany;
Max Planck Institute for Developmental Biology, D-72076 Tübingen, Germany; Instituto de Agrobiotecnología del Litoral, Universidad Nacional del Litoral - Consejo Nacional de Investigaciones Científicas y Técnicas, 3000 Santa Fe, Argentina
Proc Natl Acad Sci U S A. 2015 Nov 10;112(45):14096-101. doi: 10.1073/pnas.1512865112. Epub 2015 Oct 28.
The biogenesis of microRNAs (miRNAs), which regulate mRNA abundance through posttranscriptional silencing, comprises multiple well-orchestrated processing steps. We have identified the Arabidopsis thaliana K homology (KH) domain protein REGULATOR OF CBF GENE EXPRESSION 3 (RCF3) as a cofactor affecting miRNA biogenesis in specific plant tissues. MiRNA and miRNA-target levels were reduced in apex-enriched samples of rcf3 mutants, but not in other tissues. Mechanistically, RCF3 affects miRNA biogenesis through nuclear interactions with the phosphatases C-TERMINAL DOMAIN PHOSPHATASE-LIKE1 and 2 (CPL1 and CPL2). These interactions are essential to regulate the phosphorylation status, and thus the activity, of the double-stranded RNA binding protein and DICER-LIKE1 (DCL1) cofactor HYPONASTIC LEAVES1 (HYL1).
微小RNA（miRNA）通过转录后沉默调节mRNA丰度，其生物合成包括多个精心编排的加工步骤。我们已鉴定出拟南芥K同源（KH）结构域蛋白CBF基因表达调节剂3（RCF3）是影响特定植物组织中miRNA生物合成的辅因子。在rcf3突变体的顶端富集样本中，miRNA和miRNA靶标水平降低，但在其他组织中未降低。从机制上讲，RCF3通过与磷酸酶C末端结构域类磷酸酶1和2（CPL1和CPL2）的核相互作用影响miRNA生物合成。这些相互作用对于调节双链RNA结合蛋白和Dicer样蛋白1（DCL1）辅因子下胚轴叶片1（HYL1）的磷酸化状态以及活性至关重要。
