Farag Mohamed Ms, Sofy Ahmed R, Mousa Adel A, Ahmed Mohamed A, Alganzory Mohamed R
Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt.
Clinical Pathology Department, Military Medical Academy, Cairo, Egypt.
Virology (Auckl). 2015 Oct 12;6:1-10. doi: 10.4137/VRT.S32016. eCollection 2015.
Hepatitis C virus (HCV) infection is a major health problem recognized globally. HCV is a common cause of liver fibrosis that may lead to liver cirrhosis or hepatocellular carcinoma. The aim of this study was to estimate the prevalence of HCV infection and genotyping among Egyptian and Saudi Arabian chronic patients using different molecular techniques. HCV RNA viral load was assessed by real-time polymerase chain reaction (RT-PCR) technology. For HCV genotyping, RT-PCR hybridization fluorescence-based method and reverse hybridization line probe assay (INNO-LiPA) were used. A total of 40 anti-HCV-positive patients with chronic hepatitis C were examined for HCV RNA, genotyping, and different laboratory investigations. In the present study, HCV genotypes 4, mixed 4.1b, and 1 were detected in patients of both countries, while genotype 2 was only detected in Saudi Arabian patients. Genotyping methods for HCV showed no difference in the classification at the genotype level. With regard to HCV subtypes, INNO-LiPA assay was a reliable test in HCV genotyping for the detection of major genotypes and subtypes, while RT-PCR-based assay was a good test at the genotype level only. HCV genotype 4 was found to be the predominant genotype among Egyptian and Saudi Arabian chronic patients. In conclusion, data analysis for detecting and genotyping HCV was an important factor for understanding the epidemiology and treatment strategies of HCV among Egyptian and Saudi Arabian chronic patients.
丙型肝炎病毒（HCV）感染是全球公认的一个主要健康问题。HCV是肝纤维化的常见病因，可导致肝硬化或肝细胞癌。本研究的目的是使用不同分子技术评估埃及和沙特阿拉伯慢性病患者中HCV感染的患病率及基因分型。通过实时聚合酶链反应（RT-PCR）技术评估HCV RNA病毒载量。对于HCV基因分型，使用基于RT-PCR杂交荧光法和反向杂交线性探针分析（INNO-LiPA）。共检查了40例抗-HCV阳性的慢性丙型肝炎患者的HCV RNA、基因分型及不同实验室检查项目。在本研究中，两国患者均检测到HCV基因4型、混合4.1b型和1型，而基因2型仅在沙特阿拉伯患者中检测到。HCV基因分型方法在基因型水平的分类上没有差异。关于HCV亚型，INNO-LiPA分析在HCV基因分型中对于主要基因型和亚型的检测是一种可靠的检测方法，而基于RT-PCR的分析仅在基因型水平是一种良好的检测方法。发现HCV基因4型是埃及和沙特阿拉伯慢性病患者中的主要基因型。总之，检测和分析HCV基因分型的数据是了解埃及和沙特阿拉伯慢性病患者中HCV流行病学和治疗策略的一个重要因素。
