Chen Lingling, Kumar Sunil, Kelly Douglas, Andrews Natalie, Dallman Margaret J, French Paul M W, McGinty James
Photonics Group, Department of Physics, Imperial College London, SW7 2AZ, UK.
Institute for Chemical Biology, Department of Chemistry, Imperial College London, SW7 2AZ, UK.
Biomed Opt Express. 2014 Sep 2;5(10):3367-75. doi: 10.1364/BOE.5.003367. eCollection 2014 Oct 1.
We describe a remote focal scanning technique for optical projection tomography (OPT) implemented with an electrically tunable lens (ETL) that removes the need to scan the specimen or objective lens. Using a 4× objective lens the average spatial resolution is improved by ∼46% and the light collection efficiency by a factor of ∼6.76, thereby enabling increased acquisition speed and reduced light dose. This convenient implementation is particularly appropriate for lower magnifications and larger sample diameters where axial objective scanning would encounter problems with speed and stability.
我们描述了一种用于光学投影断层扫描（OPT）的远程聚焦扫描技术，该技术通过电可调透镜（ETL）实现，无需扫描样本或物镜。使用4倍物镜时，平均空间分辨率提高了约46%，光收集效率提高了约6.76倍，从而提高了采集速度并降低了光剂量。这种便捷的实现方式特别适用于较低放大倍数和较大样本直径的情况，在这些情况下轴向物镜扫描会遇到速度和稳定性方面的问题。
