State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
Biosens Bioelectron. 2016 Mar 15;77:799-804. doi: 10.1016/j.bios.2015.10.054. Epub 2015 Oct 20.
Au-IDE/CFP10-ESAT6 aptamer/DNA-AuNPs MSPQC for rapid detection of Mycobacterium tuberculosis was constructed based on specific detection of specific fused antigen CFP10-ESAT6 which secreted only by pathogenic M. tuberculosis in its early culture time. CFP10-ESAT6 aptamer was used as sensor specific probe of CFP10-ESAT6 antigen. Au nanoparticles (NPs) was employed to increase sensor senstivity. The Au-IDE/CFP10-ESAT6 aptamer/DNA-AuNPs electrode probe was prepared by modifying of the complementary DNA-AuNPs on to interdigital array microelectrode with CFP10-ESAT6 aptamer. CFP10-ESAT6 aptamer could specifically catch CFP10-ESAT6 protein and formed a tight complex on the electrode surface and resulted in the DNA-AuNPs fragments fell away from the electrode surface. This change can be sensitively detected by IDE-MSPQC sensor. The detection time was 96.3h. Non-pathogenic Mycobacterium did not affect detection. Compared with conventional methods, this approach was specific, more sensitive, and expected to become a valuable analysis tool for the early detection of M. tuberculosis in clinical sample.
基于特异性融合抗原 CFP10-ESAT6 的早期培养时间，仅由致病性结核分枝杆菌分泌，构建了 Au-IDE/CFP10-ESAT6 aptamer/DNA-AuNPs MSPQC 快速检测结核分枝杆菌的方法。CFP10-ESAT6 aptamer 被用作 CFP10-ESAT6 抗原的传感器特异性探针。金纳米粒子 (NPs) 用于提高传感器的灵敏度。通过在互补 DNA-AuNPs 上修饰 CFP10-ESAT6 aptamer，制备 Au-IDE/CFP10-ESAT6 aptamer/DNA-AuNPs 电极探针。CFP10-ESAT6 aptamer 可以特异性地捕获 CFP10-ESAT6 蛋白，并在电极表面形成紧密的复合物，导致 DNA-AuNPs 片段从电极表面脱落。这种变化可以通过 IDE-MSPQC 传感器灵敏地检测到。检测时间为 96.3h。非致病性分枝杆菌不会影响检测。与传统方法相比，该方法具有特异性、更高的灵敏度，有望成为临床样本中结核分枝杆菌早期检测的有价值的分析工具。
