Department of Biomedical Engineering, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Tsinghua University School of Medicine, Beijing, China.
Department of Biomedical Engineering, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Tsinghua University School of Medicine, Beijing, China; Institute of Medical Equipment, Academy of Military Medical Sciences, Tianjin, China.
Biosens Bioelectron. 2016 Mar 15;77:824-36. doi: 10.1016/j.bios.2015.10.027. Epub 2015 Oct 22.
Impedance measurement of live biological cells is widely accepted as a label free, non-invasive and quantitative analytical method to assess cell status. This method is easy-to-use and flexible for device design and fabrication. In this review, three typical techniques for impedance measurement, i.e., electric cell-substrate impedance sensing, Impedance flow cytometry and electric impedance spectroscopy, are reviewed from the aspects of theory, to electrode design and fabrication, and applications. Benefiting from the integration of microelectronic and microfluidic techniques, impedance sensing methods have expanded their applications to nearly all aspects of biology, including living cell counting and analysis, cell biology research, cancer research, drug screening, and food and environmental safety monitoring. The integration with other techniques, the fabrication of devices for certain biological assays, and the development of point-of-need diagnosis devices is predicted to be future trend for impedance sensing techniques.
活细胞的阻抗测量被广泛认为是一种无标记、非侵入性和定量分析方法，可用于评估细胞状态。这种方法易于使用，并且在设备设计和制造方面具有灵活性。在这篇综述中，从理论、电极设计和制造以及应用等方面，综述了三种典型的阻抗测量技术，即细胞-基底阻抗传感、阻抗流式细胞术和交流阻抗谱。得益于微电子和微流控技术的集成，阻抗传感方法已将其应用扩展到生物学的几乎所有方面，包括活细胞计数和分析、细胞生物学研究、癌症研究、药物筛选以及食品和环境安全监测。与其他技术的集成、用于特定生物测定的设备的制造以及即时诊断设备的开发预计将成为阻抗传感技术的未来趋势。
