Arantes Diego Antonio Costa, Costa Nádia Lago, Mendonça Elismauro Francisco, Silva Tarcília Aparecida, Batista Aline Carvalho
Department of Stomatology (Oral Pathology), Dental School, Federal University of Goiás, Goiânia, Brazil.
Department of Oral Surgery and Pathology, Dental School, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Arch Oral Biol. 2016 Jan;61:28-35. doi: 10.1016/j.archoralbio.2015.10.013. Epub 2015 Oct 22.
The aim of this study was to evaluate the expression of IL-10 and TGF-β2 in oral squamous cell carcinoma (OSCC) and its relationship with prognostic clinical and microscopic parameters.
Immunohistochemistry was used to assess the expression of IL-10 and TGF-β2 in OSCC samples from 43 patients who had undergone surgical excision and neck dissection. Metastatic lymph nodes were included in the study (n=23). Samples of healthy oral mucosa (n=20) were used as controls. The sections were evaluated using a semi-quantitative method in conjunction with staining intensity.
Our findings showed that the expression of IL-10 and TGF-β2 by neoplastic and stromal cells was high in most of the OSCC samples (>70% of samples), especially when compared to the controls (≅10% of samples) (P<0.05). OSCC neoplastic cells in cervical lymph nodes were also positive for IL-10 and TGF-β2. An association between high expression of IL-10 by neoplastic cells and advanced clinical stage (T3-T4) was verified (P=0.02). Although not statistically significant, the expression of TGF-β2 was also augmented in advanced stage tumours.
These data suggest that the ability of OSCC neoplastic cells to secrete immunosuppressive cytokines could contribute to clinical progression by maintaining a microenvironment conducive to evasion and tumour proliferation.
本研究旨在评估白细胞介素-10（IL-10）和转化生长因子-β2（TGF-β2）在口腔鳞状细胞癌（OSCC）中的表达及其与临床和微观预后参数的关系。
采用免疫组织化学法评估43例行手术切除及颈部清扫术患者的OSCC样本中IL-10和TGF-β2的表达。研究纳入了转移性淋巴结（n = 23）。取健康口腔黏膜样本（n = 20）作为对照。采用半定量方法结合染色强度对切片进行评估。
我们的研究结果显示，在大多数OSCC样本中（>70%的样本），肿瘤细胞和基质细胞中IL-10和TGF-β2的表达较高，尤其是与对照组相比（约10%的样本）（P<0.05）。颈部淋巴结中的OSCC肿瘤细胞IL-10和TGF-β2也呈阳性。证实肿瘤细胞中IL-10的高表达与临床晚期（T3 - T4）之间存在关联（P = 0.02）。尽管无统计学意义，但晚期肿瘤中TGF-β2的表达也有所增加。
这些数据表明，OSCC肿瘤细胞分泌免疫抑制细胞因子的能力可能通过维持有利于肿瘤逃逸和增殖的微环境促进临床进展。
