Zekri Abdel-Rahman N, Bakr Yasser Mabrouk, Ezzat Maali Mohamed, Zakaria Mohamed Serag Eldeen, Elbaz Tamer Mahmoud
Virology and Immunology Unit, Cancer Biology Department, National Cancer Institute, Cairo, Egypt E-mail :
Asian Pac J Cancer Prev. 2015;16(16):6923-8. doi: 10.7314/apjcp.2015.16.16.6923.
Early detection of various kinds of cancers nowadays is needed including colorectal cancer due to the highly significant effects in improving cancer treatment. The aim of this study was to evaluate the potential value of adiponectin, visfatin and resistin as early biomarkers for colorectal cancer patients.
Serum levels of adiponectin, visfatin and resistin were measured by a sandwich-enzyme-linked (ELISA) assay technique in 114 serum samples comprising 34 patients with colorectal cancer (CRC), 27 with colonic polyps (CP), 24 with inflammatory bowel disease (IBD) and 29 healthy controls .The diagnostic accuracy of each serum marker was evaluated using receiver-operating characteristic (ROC) curve analysis.
The mean concentration of adiponectin was significantly higher in CRC and CP groups than IBD and control groups (P-value <0.05). Also the mean concentration of serum resistin was significantly elevated in the IBD and control groups compared to CRC and CP groups (P-value = 0.014). However, no significant difference was noted in patients of the CRC and CP groups. On the other hand, the mean concentration of visfatin was significantly elevated in CRC and control groups compared to CP and IBD groups (P-value = 0.03). ROC analysis curves for the studied markers revealed that between CRC and IBD groups serum level of adiponectin had a sensitivity of 76.7% and a specificity of 76% at a cut off value of 3940, +LR being 3.2 and -LR 0.31 with AUC 0.852, while serum level of adiponectin between CP and IBD had a sensitivity of 77.8% and a specificity of 75% at a cut off value of 3300, with +LR=3.11 and -LR = 0.3 with AUC 0.852. On the other hand the serum level of visfatin between CRC and CP groups had a sensitivity of 65.5% and a specificity of 66.7 at a cut off value of 2.4, +LR being 1.67 and -LR 0.52 with AUC 0.698. Also the serum level of resistin had a sensitivity of 62.5% and a specificity of 70.3% at a cut off value of 24500, with +LR=2.1 and -LR = 0.53 with AUC 0.685 between control and other groups. On the other hand by comparing control vs CP groups resistin had a sensitivity of 81.8% and a specificity of 70.8% at a cut off value of 17700, with +LR=2.8 and -LR = 0.26 with AUC 0.763 while visfatin had a sensitivity of 68.2% and a specificity of 70.8% at a cut off value of 2.7, with +LR=2.34 and -LR = 0.0.45 with AUC 0.812.
These findings support potential roles of adiponectin, visfatin and resistin in early detection of CRC and discrimination of different groups of CRC, CP or IBD patients from normal healthy individuals.
由于对改善癌症治疗具有极其重要的影响，如今需要早期检测包括结直肠癌在内的各种癌症。本研究的目的是评估脂联素、内脂素和抵抗素作为结直肠癌患者早期生物标志物的潜在价值。
采用夹心酶联免疫吸附测定（ELISA）技术，检测了114份血清样本中脂联素、内脂素和抵抗素的血清水平，这些样本包括34例结直肠癌（CRC）患者、27例结肠息肉（CP）患者、24例炎症性肠病（IBD）患者和29例健康对照者。使用受试者工作特征（ROC）曲线分析评估每种血清标志物的诊断准确性。
CRC组和CP组脂联素的平均浓度显著高于IBD组和对照组（P值<0.05）。此外，与CRC组和CP组相比，IBD组和对照组血清抵抗素的平均浓度显著升高（P值=0.014）。然而，CRC组和CP组患者之间未观察到显著差异。另一方面，与CP组和IBD组相比，CRC组和对照组内脂素的平均浓度显著升高（P值=0.03）。所研究标志物的ROC分析曲线显示，在CRC组和IBD组之间，脂联素血清水平在截断值为3940时，灵敏度为76.7%，特异性为76%，阳性似然比为3.2，阴性似然比为0.31，曲线下面积（AUC）为0.852；而在CP组和IBD组之间，脂联素血清水平在截断值为3300时，灵敏度为77.8%，特异性为75%，阳性似然比为3.11，阴性似然比为0.3，AUC为0.852。另一方面，在CRC组和CP组之间，内脂素血清水平在截断值为2.4时，灵敏度为65.5%，特异性为66.7%，阳性似然比为1.67，阴性似然比为0.52，AUC为0.698。此外，抵抗素血清水平在截断值为24500时，灵敏度为62.5%，特异性为70.3%，阳性似然比为2.1，阴性似然比为0.53，对照组与其他组之间的AUC为0.685。另一方面，通过比较对照组与CP组，抵抗素在截断值为17700时，灵敏度为81.8%，特异性为70.8%，阳性似然比为2.8，阴性似然比为0.26，AUC为0.763；而内脂素在截断值为2.7时，灵敏度为68.2%，特异性为70.8%，阳性似然比为2.34，阴性似然比为0.45，AUC为0.812。
这些发现支持脂联素、内脂素和抵抗素在CRC早期检测以及区分CRC、CP或IBD不同组患者与正常健康个体方面的潜在作用。
