Rezai Omran, Khodadadi Ali, Heike Yuji, Mostafai Ali, Gerdabi Nader Dashti, Rashno Mohammad, Abdoli Zahra
Department of Immunology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran E-mail :
Asian Pac J Cancer Prev. 2015;16(16):7303-7. doi: 10.7314/apjcp.2015.16.16.7303.
Leukemia is a common cancer among children and adolescents. Wilms' tumor gene (WT1) is highly expressed in patients with acute leukemia. It is found as a tumor associated antigen (TAA) in various types of hematopoietic malignancies and can be employed as a useful marker for targeted immunotherapy and monitoring of minimal residual disease (MRD). In this regard, WT1 is a transcription factor that promotes gene activation or repression depending on cellular and promoter context. The purpose of this study was assessment of WT1 gene expression in patients with acute leukemia, measurement of IL-12 and C3 levels in serum and evaluation of the relationship between them.
We evaluated the expression of WT1 mRNA using real-time quantitative RT-PCR and serum levels of IL-12 and C3 using ELISA and nephelometry in peripheral blood of 12 newly diagnosed patients with acute leukemia and 12 controls.
The results of our study showed that the average wT1 gene expression in patients was 7.7 times higher than in healthy controls (P <0.05). In addition, IL-12 (P = 0.003) and C3 (P <0.0001) were significantly decreased in the test group compared to controls.
WT1 expression levels are significantly higher in patients compared with control subjects whereas serum levels of interleukin-12 and C3 are significantly lower in patients. Wt1 expression levels in patients are inversely related with serum levels of IL-12 and C3.
白血病是儿童和青少年中常见的癌症。威尔姆斯瘤基因（WT1）在急性白血病患者中高表达。它在各种类型的造血系统恶性肿瘤中作为肿瘤相关抗原（TAA）被发现，并且可作为靶向免疫治疗和微小残留病（MRD）监测的有用标志物。在这方面，WT1是一种转录因子，根据细胞和启动子环境促进基因激活或抑制。本研究的目的是评估急性白血病患者中WT1基因的表达，测量血清中IL-12和C3水平并评估它们之间的关系。
我们使用实时定量RT-PCR评估WT1 mRNA的表达，并使用ELISA和比浊法测量12例新诊断的急性白血病患者和12例对照者外周血中IL-12和C3的血清水平。
我们的研究结果表明，患者中WT1基因的平均表达比健康对照高7.7倍（P <0.05）。此外，与对照组相比，试验组中IL-12（P = 0.003）和C3（P <0.0001）显著降低。
与对照受试者相比，患者中WT1表达水平显著更高，而患者中白细胞介素-12和C3的血清水平显著更低。患者中WT1表达水平与IL-12和C3的血清水平呈负相关。
