School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, China.
Centre for Pharmacognosy and Phytotherapy, Research Cluster Biodiversity and Medicines, UCL School of Pharmacy, University of London, UK.
Phytother Res. 2015 Dec;29(12):1974-81. doi: 10.1002/ptr.5492. Epub 2015 Oct 30.
This study aims to investigate the additive or synergistic effects and mechanism of intestinal absorption of extracts from two commonly used 'dispelling-wind' TCM botanical drugs [roots of Angelica dahurica (Hoffm.) Benth. & Hook. f. ex Franch. & Sav. (RAD) and Saposhnikovia divaricata (Turcz.) Schischk. (RSD)] using chlorogenic acid as a marker substance. Ex vivo everted intestinal sac and in situ single pass perfusion methods using rats were employed to investigate the effects of two TCM botanical drugs extracts on the intestinal absorption of chlorogenic acid. Both the extracts of RAD and RSD showed synergistic properties on the intestinal absorption of chlorogenic acid. The verapamil (a P-gp inhibitor) and intestinal dysbacteriosis model induced by norfloxacin increased the P(app) and K(a) of intestinal absorption of chlorogenic acid. These synergistic effects on intestinal absorption in a rat model can be correlated with the inhibition of P-gp and regulation of gut microbiota. This experimental approach has helped to better understand changes in the absorption of chlorogenic acid under different conditions.
本研究旨在以绿原酸为标记物，研究两种常用“祛风”中药植物药（白芷根[Angelica dahurica （Hoffm.）Benth. & Hook. f. ex Franch. & Sav. （RAD）]和防风[Saposhnikovia divaricata （Turcz.）Schischk. （RSD）]的提取物的协同或相加作用及其肠吸收机制。采用外翻肠囊和在体单向灌流方法，研究两种中药植物药提取物对绿原酸肠吸收的影响。RAD 和 RSD 的提取物对绿原酸的肠吸收均表现出协同作用。维拉帕米（P-糖蛋白抑制剂）和诺氟沙星诱导的肠道菌群失调模型增加了绿原酸肠吸收的 Papp 和 Ka。在大鼠模型中，这种对肠吸收的协同作用可能与 P-糖蛋白的抑制和肠道微生物群的调节有关。这种实验方法有助于更好地理解在不同条件下绿原酸吸收的变化。
