Ma Hongyu, Wang Zedong, Wang Chengdong, Li Caiwu, Wei Feng, Liu Quan
College of Life Science, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, Jilin Province, China - Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Military Veterinary Institute, Academy of Military Medical Sciences, Changchun, China.
Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Military Veterinary Institute, Academy of Military Medical Sciences, Changchun, China.
Parasite. 2015;22:30. doi: 10.1051/parasite/2015030. Epub 2015 Oct 29.
Toxoplasma gondii can infect nearly all warm-blooded animals. We report an acute fatal T. gondii infection in the endangered giant panda (Ailuropoda melanoleuca) in a zoo in China, characterized by acute gastroenteritis and respiratory symptoms. T. gondii infection was confirmed by immunological and molecular methods. Multilocus nested PCR-RFLP revealed clonal type I at the SAG1 and c29-2 loci, clonal type II at the SAG2, BTUB, GRA6, c22-8, and L358 loci, and clonal type III at the alternative SAG2 and SAG3 loci, thus, a potential new genotype of T. gondii in the giant panda. Other possible pathogens were not detected. To our knowledge, this is the first report of clinical toxoplasmosis in a giant panda.
刚地弓形虫可感染几乎所有温血动物。我们报告了中国一家动物园内濒危大熊猫（大熊猫）发生的一例急性致命性刚地弓形虫感染，其特征为急性肠胃炎和呼吸道症状。通过免疫学和分子方法确诊了刚地弓形虫感染。多位点巢式PCR-RFLP分析显示，在SAG1和c29-2位点为克隆I型，在SAG2、BTUB、GRA6、c22-8和L358位点为克隆II型，在替代的SAG2和SAG3位点为克隆III型，因此，这是大熊猫体内刚地弓形虫的一种潜在新基因型。未检测到其他可能的病原体。据我们所知，这是大熊猫临床弓形虫病的首例报告。
