Miao Hong, Zhong Dan, Zhou Zinan, Yang Xiaoming
College of Pharmaceutical Sciences, Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, Southwest University, Chongqing 400715, China.
Nanoscale. 2015 Dec 7;7(45):19066-72. doi: 10.1039/c5nr05362e. Epub 2015 Oct 30.
Herein, papain-functionalized Cu nanoclusters (CuNCs@Papain) were originally synthesized in aqueous solution together with a quantum yield of 14.3%, and showed obviously red fluorescence at 620 nm. Meanwhile, their corresponding fluorescence mechanism was fully elucidated by fluorescence spectroscopy, HR-TEM, FTIR spectroscopy, and XPS. Subsequently, the as-prepared CuNCs were employed as probes for detecting H2O2. Using CuNCs as probes, H2O2 was determined in the range from 1 μM to 50 μM based on a linear decrease of fluorescence intensity as well as a detection limit of 0.2 μM with a signal-to-noise ratio of 3. More significantly, it has been proved that CuNCs could convert H2O2 to ˙OH, which exhibited dramatic antibacterial activity. Both in vitro and in vivo experiments were performed to validate their antibacterial activity against Gram-positive/negative bacteria and actual wound infection, suggesting their potential for serving as one type of promising antibacterial material.
在此，木瓜蛋白酶功能化的铜纳米团簇（CuNCs@Papain）最初在水溶液中合成，量子产率为14.3%，并在620nm处显示出明显的红色荧光。同时，通过荧光光谱、高分辨透射电子显微镜（HR-TEM）、傅里叶变换红外光谱（FTIR）和X射线光电子能谱（XPS）全面阐明了其相应的荧光机制。随后，将制备好的CuNCs用作检测过氧化氢（H2O2）的探针。以CuNCs为探针，基于荧光强度的线性降低，在1μM至50μM范围内测定了H2O2，检测限为0.2μM，信噪比为3。更重要的是，已证明CuNCs可将H2O2转化为˙OH，其具有显著的抗菌活性。进行了体外和体内实验，以验证其对革兰氏阳性/阴性细菌和实际伤口感染的抗菌活性，表明它们有潜力作为一种有前景的抗菌材料。
