Mesbahzadeh Behzad, Akbari Mohsen, Kor Nasroallah Moradi, Zadeh Jalal Bayati
Atherosclerosis and Coronary Artery Research Center, Department of Physiology, Birjand University of Medical Sciences, Birjand, Iran.
Department of Animal Sciences, College of Agriculture and Natural Resources, Razi University, Kermanshah, Iran.
Electron Physician. 2015 Oct 19;7(6):1376-80. doi: 10.14661/1376. eCollection 2015 Oct.
Peppermint is an efficient medicinal plant for the treatment of diseases, and it also can be used to produce raw materials in the pharmaceutical industry. The purpose of the current study was to evaluate the effects of various levels of peppermint alcoholic extract on body-weight gain and blood biochemical parameters in adult male Wistar rats.
This experiment was conducted using a completely randomized design (CRD). Fifty adult, healthy, male Wistar rats (ages of 2.5-3 months; weights of 190-210 g) were allocated randomly into five groups. T1 was the control group in which the rats received 0.3 ml of distilled water). Groups T2, T3, T4, and T5 received 75, 150, 300, and 600 mg/kg of peppermint extract, respectively. The rats received daily pretreatment by oral gavages for 21 days. We recorded body weights at the beginning and at the end of the study to determine the changes in the body weights. Blood samples were collected for the measurement of glucose, cholesterol, triglycerides, HDL, LDL, albumin, globulin, and total protein. Statistical analysis of the data was done by SAS software. The data statistically analyzed using one-way analysis of variance (ANOVA), which was conducted through Dennett's multiple comparison post-test.
The results indicated that the rats treated with peppermint gained more weight (p < 0.05) and also decreased the serum concentrations of triglycerides, total cholesterol, LDL, and glucose in T3, T4 and T5 than the other groups (p < 0.05).
Peppermint extract had a positive effect on body-weight gain and some blood parameters in adult male Wistar rats. The findings showed that peppermint is a crucial substance at high temperature, and future research should be focused on determining the details of the mechanisms involved in producing the observed effects of peppermint extract.
薄荷是一种治疗疾病的高效药用植物，也可用于制药工业生产原料。本研究的目的是评估不同水平的薄荷醇提取物对成年雄性Wistar大鼠体重增加和血液生化参数的影响。
本实验采用完全随机设计（CRD）。将50只成年、健康、雄性Wistar大鼠（年龄2.5 - 3个月；体重190 - 210克）随机分为五组。T1为对照组，大鼠接受0.3毫升蒸馏水。T2、T3、T4和T5组分别接受75、150、300和600毫克/千克的薄荷提取物。大鼠每天经口灌胃预处理21天。在研究开始和结束时记录体重，以确定体重变化。采集血样用于测量葡萄糖、胆固醇、甘油三酯、高密度脂蛋白、低密度脂蛋白、白蛋白、球蛋白和总蛋白。数据的统计分析由SAS软件完成。使用单因素方差分析（ANOVA）对数据进行统计分析，并通过Dennett多重比较后检验进行。
结果表明，用薄荷处理的大鼠体重增加更多（p < 0.05），并且T3、T4和T5组的甘油三酯、总胆固醇、低密度脂蛋白和葡萄糖的血清浓度比其他组降低（p < 0.05）。
薄荷提取物对成年雄性Wistar大鼠的体重增加和一些血液参数有积极影响。研究结果表明，薄荷在高温下是一种关键物质，未来的研究应集中于确定薄荷提取物产生观察到的效果所涉及机制的细节。
