共聚焦扫描显微镜荧光三维成像
Three-dimensional imaging in fluorescence by confocal scanning microscopy.
作者信息
Brakenhoff G J, van der Voort H T, van Spronsen E A, Nanninga N
机构信息
Department of Electrical Microscopy and Molecular Cytology, University of Amsterdam, The Netherlands.
出版信息
J Microsc. 1989 Feb;153(Pt 2):151-9. doi: 10.1111/j.1365-2818.1989.tb00555.x.
Abstract
The improved resolution and sectioning capability of a confocal microscope make it an ideal instrument for extracting three-dimensional information especially from extended biological specimens. The imaging properties, also with finite detection pinholes are considered and a number of biological applications demonstrated.
摘要
共聚焦显微镜分辨率和切片能力的提升使其成为从扩展生物样本中提取三维信息的理想仪器。文中考虑了具有有限检测针孔的成像特性，并展示了一些生物学应用。
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