Papworth G D, Delaney P M, Bussau L J, Vo L T, King R G
Department of Pharmacology, Monash University, Clayton, Victoria, Australia.
J Anat. 1998 May;192 ( Pt 4)(Pt 4):489-95. doi: 10.1046/j.1469-7580.1998.19240489.x.
A fluorescence confocal microscopy technique was employed to obtain subsurface images of nerve and microvascular structure in the vas deferens and colon of the living rat. The use of dual labelling with vital dyes and 2-channel confocal acquisition allowed differentiation of microscopic structure at both low and higher magnification. Characteristic staining patterns of nerves and blood vessels were repeatedly obtained in each tissue, suggesting the potential of this technique for studying morphological changes associated with surgical procedures and/or models of neuronal or vascular pathology.
采用荧光共聚焦显微镜技术获取活体大鼠输精管和结肠中神经和微血管结构的亚表面图像。使用活性染料进行双重标记和双通道共聚焦采集，能够在低倍和高倍放大下区分微观结构。在每个组织中反复获得神经和血管的特征性染色模式，表明该技术在研究与手术操作和/或神经元或血管病理学模型相关的形态学变化方面具有潜力。
