Wang Jing, Zhang Kaichun, Zhang Xiaoming, Yan Guohua, Zhou Yu, Feng Laibao, Ni Yang, Duan Xuwei
Institute of Forestry and Pomology, Beijing Academy of Agricultural and Forestry Sciences, Beijing, 100093, China; Beijing Engineering Research Center for Deciduous Fruit Trees, Beijing, 100093, China; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, P.R. China, Beijing, 100093, China.
Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Xiangshan Nanxincun 20, Beijing, 100093, China.
PLoS One. 2015 Oct 30;10(10):e0141261. doi: 10.1371/journal.pone.0141261. eCollection 2015.
A cross between the sweet cherry (Prunus avium) cultivars 'Wanhongzhu' and 'Lapins' was performed to create a mapping population suitable for the construction of a linkage map. The specific-locus amplified fragment (SLAF) sequencing technique used as a single nucleotide polymorphism (SNP) discovery platform and generated 701 informative genotypic assays; these, along with 16 microsatellites (SSRs) and the incompatibility (S) gene, were used to build a map which comprised 8 linkage groups (LGs) and covered a genetic distance of 849.0 cM. The mean inter-marker distance was 1.18 cM and there were few gaps > 5 cM in length. Marker collinearity was maintained with the established peach genomic sequence. The map was used to show that trunk diameter (TD) is under the control of 4 loci, mapping to 3 different LGs. Different locus influenced TD at a varying stage of the tree's development. The high density 'W×L' genetic linkage map has the potential to enable high-resolution identification of QTLs of agronomically relevant traits, and accelerate sweet cherry breeding.
将甜樱桃（Prunus avium）品种‘万红珠’和‘拉宾斯’进行杂交，以创建一个适合构建连锁图谱的作图群体。采用特异位点扩增片段（SLAF）测序技术作为单核苷酸多态性（SNP）发现平台，产生了701个信息性基因型分析；这些分析连同16个微卫星（SSR）和不亲和性（S）基因一起用于构建一个包含8个连锁群（LG）、覆盖遗传距离849.0 cM的图谱。标记间平均距离为1.18 cM，长度大于5 cM的间隙很少。标记共线性与已建立的桃基因组序列保持一致。该图谱用于表明树干直径（TD）受4个位点控制，定位于3个不同的LG。不同位点在树发育的不同阶段影响TD。高密度的‘W×L’遗传连锁图谱有潜力实现对农艺相关性状QTL的高分辨率鉴定，并加速甜樱桃育种。
