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不同组织来源的干细胞：神经分化能力的比较

Different Tissue-Derived Stem Cells: A Comparison of Neural Differentiation Capability.

作者信息

Bonaventura Gabriele, Chamayou Sandrine, Liprino Annalisa, Guglielmino Antonino, Fichera Michele, Caruso Massimo, Barcellona Maria Luisa

机构信息

Department of Pharmaceutical Science, Biochemistry Section, University of Catania, Catania, Italy; Institute of Neurological Sciences, Italian National Research Council, Catania, Italy.

Unità di Medicina della Riproduzione, Fondazione Hera, Sant'Agata Li Battiati (CT), Italy.

出版信息

PLoS One. 2015 Oct 30;10(10):e0140790. doi: 10.1371/journal.pone.0140790. eCollection 2015.

DOI:10.1371/journal.pone.0140790
PMID:26517263
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4627815/
Abstract

BACKGROUND

Stem cells are capable of self-renewal and differentiation into a wide range of cell types with multiple clinical and therapeutic applications. Stem cells are providing hope for many diseases that currently lack effective therapeutic methods, including strokes, Huntington's disease, Alzheimer's and Parkinson's disease. However, the paucity of suitable cell types for cell replacement therapy in patients suffering from neurological disorders has hampered the development of this promising therapeutic approach.

AIM

The innovative aspect of this study has been to evaluate the neural differentiation capability of different tissue-derived stem cells coming from different tissue sources such as bone marrow, umbilical cord blood, human endometrium and amniotic fluid, cultured under the same supplemented media neuro-transcription factor conditions, testing the expression of neural markers such as GFAP, Nestin and Neurofilaments using the immunofluorescence staining assay and some typical clusters of differentiation such as CD34, CD90, CD105 and CD133 by using the cytofluorimetric test assay.

RESULTS

Amniotic fluid derived stem cells showed a more primitive phenotype compared to the differentiating potential demonstrated by the other stem cell sources, representing a realistic possibility in the field of regenerative cell therapy suitable for neurodegenerative diseases.

摘要

背景

干细胞能够自我更新并分化为多种细胞类型，具有多种临床和治疗应用。干细胞为目前缺乏有效治疗方法的许多疾病带来了希望，包括中风、亨廷顿舞蹈症、阿尔茨海默病和帕金森病。然而，对于患有神经疾病的患者，用于细胞替代治疗的合适细胞类型匮乏，阻碍了这种有前景的治疗方法的发展。

目的

本研究的创新之处在于评估来自不同组织来源（如骨髓、脐带血、人子宫内膜和羊水）的不同组织源性干细胞在相同添加了神经转录因子的培养基条件下培养时的神经分化能力，使用免疫荧光染色法检测神经标志物（如胶质纤维酸性蛋白、巢蛋白和神经丝蛋白）的表达，并使用细胞荧光测定法检测一些典型的分化簇（如CD34、CD90、CD105和CD133）。

结果

与其他干细胞来源所显示的分化潜能相比，羊水来源的干细胞表现出更原始的表型，这在适用于神经退行性疾病的再生细胞治疗领域代表了一种现实的可能性。

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